Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution.
Zhang Y,
Zhang Y,
Liu ZY,
Cheng ML,
Ma J,
Wang Y,
Qin CF,
Fang X
EMBO Rep
20(11):e47016
(2019 Nov 5)
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Sample: |
Xrn1 resistance RNA2 from Zika virus monomer, 22 kDa Zika virus RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 9
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|
RgGuinier |
2.4 |
nm |
Dmax |
8.5 |
nm |
VolumePorod |
34 |
nm3 |
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Sample: |
Xrn1 resistance RNA1 from Dengue virus 2 monomer, 21 kDa Dengue virus 2 RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 9
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RgGuinier |
2.2 |
nm |
Dmax |
8.0 |
nm |
VolumePorod |
31 |
nm3 |
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Sample: |
Xrn1 resistance RNA1-2 from Dengue virus 2 monomer, 46 kDa Dengue virus 2 RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Apr 2
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RgGuinier |
3.9 |
nm |
Dmax |
13.4 |
nm |
VolumePorod |
67 |
nm3 |
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Sample: |
Xrn1 resistance RNA-1 from West Nile virus monomer, 25 kDa West Nile virus RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 9
|
|
RgGuinier |
2.4 |
nm |
Dmax |
8.4 |
nm |
VolumePorod |
34 |
nm3 |
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Sample: |
Xrn1 resistance RNA-1 from Murray Valley Encephalitis monomer, 26 kDa Murray Valley Encephalitis RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 9
|
|
RgGuinier |
2.4 |
nm |
Dmax |
8.8 |
nm |
VolumePorod |
31 |
nm3 |
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Sample: |
3'SL from Dengue virus 2 monomer, 31 kDa Dengue virus 2 RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Feb 24
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RgGuinier |
3.6 |
nm |
Dmax |
14.1 |
nm |
VolumePorod |
40 |
nm3 |
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|
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Sample: |
Xrn1 resistance RNA1-2 from Dengue virus 2 monomer, 46 kDa Dengue virus 2 RNA
|
Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 9
|
|
RgGuinier |
3.5 |
nm |
Dmax |
12.5 |
nm |
VolumePorod |
65 |
nm3 |
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Sample: |
Xrn1 resistance RNA2 from Zika virus monomer, 22 kDa Zika virus RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2017 Sep 11
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RgGuinier |
2.1 |
nm |
Dmax |
7.2 |
nm |
VolumePorod |
29 |
nm3 |
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Sample: |
Xrn1 resistance RNA1-2 from West Nile virus monomer, 75 kDa West Nile virus RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Apr 2
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RgGuinier |
4.7 |
nm |
Dmax |
17.4 |
nm |
VolumePorod |
235 |
nm3 |
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Sample: |
SL3 from West Nile virus monomer, 23 kDa West Nile virus RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2017 Jun 27
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|
RgGuinier |
2.8 |
nm |
Dmax |
9.5 |
nm |
VolumePorod |
29 |
nm3 |
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Sample: |
Xrn1 resistance RNA2 from Dengue virus 2 monomer, 21 kDa Dengue virus 2 RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Mar 16
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RgGuinier |
2.2 |
nm |
Dmax |
7.6 |
nm |
VolumePorod |
29 |
nm3 |
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Sample: |
DB12 from Dengue virus 2 monomer, 56 kDa Dengue virus 2 RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Feb 24
|
|
RgGuinier |
4.7 |
nm |
Dmax |
16.2 |
nm |
VolumePorod |
96 |
nm3 |
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Sample: |
Xrn1 resistance RNA2 from West Nile virus monomer, 23 kDa West Nile virus RNA
|
Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2017 Apr 7
|
|
RgGuinier |
2.2 |
nm |
Dmax |
7.7 |
nm |
VolumePorod |
30 |
nm3 |
|
|
|
|
|
Sample: |
Xrn1 resistance RNA2 from Murray Valley Encephalitis monomer, 22 kDa Murray Valley Encephalitis RNA
|
Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 14
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|
RgGuinier |
2.2 |
nm |
Dmax |
8.2 |
nm |
VolumePorod |
28 |
nm3 |
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|
Sample: |
DB12 from Zika virus monomer, 47 kDa Zika virus RNA
|
Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Apr 2
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|
RgGuinier |
3.3 |
nm |
Dmax |
11.2 |
nm |
VolumePorod |
92 |
nm3 |
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Sample: |
DB12 from West Nile virus monomer, 59 kDa West Nile virus RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jan 19
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|
RgGuinier |
3.9 |
nm |
Dmax |
13.4 |
nm |
VolumePorod |
160 |
nm3 |
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Sample: |
3'SL from Zika virus monomer, 32 kDa Zika virus RNA
|
Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 9
|
|
RgGuinier |
3.9 |
nm |
Dmax |
14.1 |
nm |
VolumePorod |
52 |
nm3 |
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Sample: |
3'SL from West Nile virus monomer, 31 kDa West Nile virus RNA
|
Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2017 Apr 7
|
|
RgGuinier |
3.5 |
nm |
Dmax |
13.2 |
nm |
VolumePorod |
39 |
nm3 |
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Sample: |
Subgenomic flavivirus RNA from Zika virus monomer, 133 kDa Zika virus RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Mar 13
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|
RgGuinier |
7.5 |
nm |
Dmax |
29.6 |
nm |
VolumePorod |
230 |
nm3 |
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|
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Sample: |
Subgenomic flavivirus RNAs from Dengue virus 2 monomer, 137 kDa Dengue virus 2 RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Dec 16
|
|
RgGuinier |
7.9 |
nm |
Dmax |
30.5 |
nm |
VolumePorod |
236 |
nm3 |
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|
|
|
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Sample: |
Subgenomic flavivirus RNAs from West nile virus monomer, 170 kDa West Nile virus RNA
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Buffer: |
20mM Tris-HCl, 100mM NaCl, 5mM MgCl2, pH: 7.5 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Mar 13
|
|
RgGuinier |
7.9 |
nm |
Dmax |
31.8 |
nm |
VolumePorod |
261 |
nm3 |
|
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