Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.

Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG
Structure (2019 Oct 5)

PMID: 31601460 doi: 10.1016/j.str.2019.09.007

Submitted to SASBDB: 2019 Aug 17 Published in SASBDB: 2019 Sep 16

SASDG64 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models) Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15 RgGuinier 5.5 nm Dmax 26.0 nm VolumePorod 194 nm3

SASDG94 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models) Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15 RgGuinier 5.5 nm Dmax 26.0 nm VolumePorod 194 nm3

SASDGA4 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (parallel CORAL and MultiFoXS models) Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15 RgGuinier 5.5 nm Dmax 26.0 nm VolumePorod 194 nm3

SASDG74 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models) Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15 RgGuinier 5.3 nm Dmax 25.7 nm VolumePorod 168 nm3

SASDGB4 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models) Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15 RgGuinier 5.1 nm Dmax 25.7 nm VolumePorod 168 nm3

SASDGC4 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (parallel CORAL and MultiFoXS models) Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15 RgGuinier 5.1 nm Dmax 25.7 nm VolumePorod 168 nm3

SASDG84 – Autoinhibited dimer of truncated 6xHis Cytohesin-2 (ARNO, amino acids 2-400) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF, GASBOR and antiparallel CORAL models) Sample: Cytohesin-2 dimer, 95 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15 RgGuinier 5.3 nm Dmax 27.0 nm VolumePorod 180 nm3