Integrated Structural Modeling of Full-Length LRH-1 Reveals Inter-domain Interactions Contribute to Receptor Structure and Function.

Seacrist CD, Kuenze G, Hoffmann RM, Moeller BE, Burke JE, Meiler J, Blind RD
Structure (2020 May 19)
PMID: 32433991
doi: 10.1016/j.str.2020.04.020 		Submitted to SASBDB: 2019 Sep 11
Published in SASBDB:

SASDG85 – Wild-type full-length Liver Receptor Homolog-1/Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha peptide complex bound to the CYP7A1 promoter oligonucleotide duplex.

Liver Receptor Homolog-1Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 AlphaCYP7A1 Promoter ForwardCYP7A1 Promoter Reverse experimental SAS data
Liver Receptor Homolog-1 Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha CYP7A1 Promoter Forward CYP7A1 Promoter Reverse Kratky plot
Sample: Liver Receptor Homolog-1 monomer, 64 kDa Homo sapiens protein
Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha monomer, 2 kDa Homo sapiens protein
CYP7A1 Promoter Forward monomer, 4 kDa Homo sapiens DNA
CYP7A1 Promoter Reverse monomer, 4 kDa Homo sapiens DNA
Buffer: 20 mM TRIS, 150 mM NaCl, 2% v/v glycerol, 0.5 mM CHAPS, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 26
RgGuinier 3.8 nm
Dmax 13.0 nm
VolumePorod 76 nm3