The dual PDZ domain from Postsynaptic density protein 95 forms a scaffold with peptide ligand

Rodzli N, Lockhart-Cairns M, Levy C, Chipperfield J, Bird L, Baldock C, Prince S
Biophysical Journal (2020 Jun)

doi: 10.1016/j.bpj.2020.06.018

Submitted to SASBDB: 2019 Sep 16 Published in SASBDB: 2019 Sep 20

SASDGB5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) bound to RRESEI peptide Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein Buffer: 20 mM TRIS/HCl, 150 mM NaCl + 10 mM RRESEI, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 11 RgGuinier 3.0 nm Dmax 13.1 nm VolumePorod 32 nm3

SASDGC5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) bound to RRESEI peptide (diluted) Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein Buffer: 20 mM TRIS/HCl, 150 mM NaCl + 5 mM RRESEI, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 11 RgGuinier 2.6 nm Dmax 12.4 nm VolumePorod 32 nm3

SASDGD5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) bound to RRESEI peptide (Paused SEC) Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein Buffer: 20 mM TRIS/HCl, 150 mM NaCl + 10 mM RRESEI, pH: 8.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jan 26 RgGuinier 2.3 nm Dmax 6.8 nm VolumePorod 28 nm3

SASDGE5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein Buffer: 20 mM TRIS/HCl, 150 mM NaCl, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 11 RgGuinier 2.4 nm Dmax 9.2 nm VolumePorod 32 nm3

SASDGF5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) (diluted) Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein Buffer: 20 mM TRIS/HCl, 150 mM NaCl, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 11 RgGuinier 2.5 nm Dmax 9.5 nm VolumePorod 32 nm3

SASDGG5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) (Paused SEC) Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein Buffer: 20 mM TRIS/HCl, 150 mM NaCl, pH: 8.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jan 26 RgGuinier 2.4 nm Dmax 6.4 nm VolumePorod 31 nm3

SASDGH5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) + glutathione (GSH) Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein Buffer: 20 mM TRIS/HCl, 150 mM NaCl + 10 mM GSH, pH: 8.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Sep 16 RgGuinier 2.7 nm Dmax 6.3 nm VolumePorod 29 nm3

SASDGJ5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) + glutathione (GSH) (diluted) Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein Buffer: 20 mM TRIS/HCl, 150 mM NaCl + 16 mM GSH, pH: 8.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Sep 16 RgGuinier 3.1 nm Dmax 7.0 nm VolumePorod 41 nm3

SASDGK5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) + glutathione (GSH) (Paused SEC) Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein Buffer: 20 mM TRIS/HCl, 150 mM NaCl + 10 mM GSH, pH: 8.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jan 26 RgGuinier 2.4 nm Dmax 7.2 nm VolumePorod 30 nm3