Asparagine-84, a regulatory allosteric site residue, helps maintain the quaternary structure of Campylobacter jejuni dihydrodipicolinate synthase.
Majdi Yazdi M,
Saran S,
Mrozowich T,
Lehnert C,
Patel TR,
Sanders DAR,
Palmer DRJ
J Struct Biol
:107409
(2019 Oct 31)
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Sample: |
4-hydroxy-tetrahydrodipicolinate synthase tetramer, 131 kDa Campylobacter jejuni protein
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Buffer: |
20 mM Tris-HCl, 150 mM NaCl, pH: 8 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2017 Aug 2
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RgGuinier |
3.4 |
nm |
Dmax |
9.0 |
nm |
VolumePorod |
188 |
nm3 |
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Sample: |
4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant) dimer, 65 kDa Campylobacter jejuni protein
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Buffer: |
20 mM Tris-HCl, 150 mM NaCl, pH: 8 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2017 Aug 2
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|
RgGuinier |
3.1 |
nm |
Dmax |
9.5 |
nm |
VolumePorod |
108 |
nm3 |
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Sample: |
4-hydroxy-tetrahydrodipicolinate synthase (N84A mutant), 33 kDa Campylobacter jejuni protein
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Buffer: |
20 mM Tris-HCl, 150 mM NaCl, pH: 8 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2017 Aug 2
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RgGuinier |
3.3 |
nm |
Dmax |
8.9 |
nm |
VolumePorod |
163 |
nm3 |
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