Combination of SAXS and Protein Painting Discloses the Three-Dimensional Organization of the Bacterial Cysteine Synthase Complex, a Potential Target for Enhancers of Antibiotic Action.

Rosa B, Marchetti M, Paredi G, Amenitsch H, Franko N, Benoni R, Giabbai B, De Marino MG, Mozzarelli A, Ronda L, Storici P, Campanini B, Bettati S
Int J Mol Sci 20(20) (2019 Oct 21)

PMID: 31640223 doi: 10.3390/ijms20205219

Submitted to SASBDB: 2019 Oct 12 Published in SASBDB: 2019 Oct 25

SASDGV6 – Cysteine synthase A Sample: Cysteine synthase A dimer, 71 kDa Escherichia coli protein Buffer: 20 mM sodium phosphate, 85 mM NaCl, 2 mM EDTA, 10 mM 2-MCE, pH: 7.5 Experiment: SAXS data collected at Austrian SAXS beamline 5.2L, ELETTRA on 2016 Jun 1 RgGuinier 2.6 nm Dmax 8.5 nm VolumePorod 108 nm3

SASDGW6 – Serine acetyltransferase Sample: Serine acetyltransferase hexamer, 177 kDa Escherichia coli protein Buffer: 20 mM sodium phosphate, 85 mM NaCl, 2 mM EDTA, 10 mM 2-MCE, pH: 7.5 Experiment: SAXS data collected at Austrian SAXS beamline 5.2L, ELETTRA on 2016 Jun 1 RgGuinier 3.9 nm Dmax 13.0 nm VolumePorod 280 nm3

SASDGX6 – Cysteine synthase/Serine acetyltransferase complex Sample: Cysteine synthase A (4-mer) tetramer, 143 kDa Escherichia coli protein Serine acetyltransferase (6-mer) hexamer, 177 kDa Escherichia coli protein Buffer: 20 mM sodium phosphate, 85 mM NaCl, 2 mM EDTA, 10 mM 2-MCE, pH: 7.5 Experiment: SAXS data collected at Austrian SAXS beamline 5.2L, ELETTRA on 2016 Jun 1 RgGuinier 6.1 nm Dmax 22.0 nm VolumePorod 457 nm3