Salt Dependence of A-Form RNA Duplexes: Structures and Implications.

Chen YL, Pollack L
J Phys Chem B 123(46):9773-9785 (2019 Nov 21)

PMID: 31638810 doi: 10.1021/acs.jpcb.9b07502

Submitted to SASBDB: 2019 Nov 6 Published in SASBDB: 2019 Nov 8

SASDHG2 – 12 base-paired RNA double helix (RNA12) with 30 mM KCl - SWAXS Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA Buffer: 30 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16 RgGuinier 1.4 nm

SASDHH2 – 12 base-paired RNA double helix (RNA12) with 50 mM KCl - SWAXS Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA Buffer: 50 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16 RgGuinier 1.4 nm

SASDHJ2 – 12 base-paired RNA double helix (RNA12) with 100 mM KCl - SWAXS Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA Buffer: 100 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16 RgGuinier 1.5 nm

SASDHK2 – 12 base-paired RNA double helix (RNA12) with 200 mM KCl - SWAXS Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA Buffer: 200 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16 RgGuinier 1.6 nm

SASDHL2 – 12 base-paired RNA double helix (RNA12) with 500 mM KCl - SWAXS Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA Buffer: 500 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16 RgGuinier 2.1 nm

SASDHM2 – 12 base-paired RNA double helix (RNA12) with 0.25 mM MgCl2 - SWAXS Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA Buffer: 0.25 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16 RgGuinier 1.4 nm

SASDHN2 – 12 base-paired RNA double helix (RNA12) with 1 mM MgCl2 - SWAXS Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA Buffer: 1.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16 RgGuinier 1.6 nm

SASDHP2 – 12 base-paired RNA double helix (RNA12) with 5 mM MgCl2 - SWAXS Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA Buffer: 5.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16 RgGuinier 2.0 nm

SASDHQ2 – 25 base-paired RNA double helix (RNA25) with 100 mM NaCl - WAXS Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA Buffer: 100 mM NaCl, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13 RgGuinier 2.1 nm

SASDHR2 – 25 base-paired RNA double helix (RNA25) with 10 mM MgCl2 - WAXS Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA Buffer: 10.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13 RgGuinier 2.6 nm

SASDHS2 – 25 base-paired RNA double helix (RNA25) with 400 mM KCl - WAXS Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA Buffer: 400 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13 RgGuinier 2.3 nm

SASDHT2 – 25 base-paired DNA double helix (DNA25) with 400 mM KCl - SWAXS Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA Buffer: 400 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16 RgGuinier 1.9 nm

SASDHU2 – 25 base-paired DNA double helix (DNA25) with 0.5 mM MgCl2 - SWAXS Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA Buffer: 0.5 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16 RgGuinier 2.0 nm

SASDHV2 – 25 base-paired DNA double helix (DNA25) with 2 mM MgCl2 - SWAXS Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA Buffer: 2.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16 RgGuinier 1.9 nm