Mechanism of Epac activation: structural and functional analyses of Epac2 hinge mutants with constitutive and reduced activities.

Tsalkova T, Blumenthal DK, Mei FC, White MA, Cheng X
J Biol Chem 284(35):23644-51 (2009 Aug 28)

PMID: 19553663 doi: 10.1074/jbc.M109.024950

Submitted to SASBDB: 2019 Oct 16 Published in SASBDB: 2019 Nov 14

SASDH62 – Isoform 3 of Rap guanine nucleotide exchange factor 4, aE2F-DB08 Sample: Isoform 3 of Rap guanine nucleotide exchange factor 4 monomer, 114 kDa Mus musculus protein Buffer: 150 mM NaCl, 1 mM EDTA, 1 mM DTT, and 10 mM Tris-HCl, pH: 7.5 Experiment: SAXS data collected at Anton Paar SAXSess, University of Utah on 2008 Aug 7 RgGuinier 3.2 nm Dmax 10.7 nm VolumePorod 123 nm3

SASDH72 – Isoform 3 of Rap guanine nucleotide exchange factor 4, aE2G-DB08 Sample: Isoform 3 of Rap guanine nucleotide exchange factor 4 monomer, 114 kDa Mus musculus protein Buffer: 150 mM NaCl, 1 mM EDTA, 1 mM DTT, and 10 mM Tris-HCl, pH: 7.5 Experiment: SAXS data collected at Anton Paar SAXSess, University of Utah on 2008 Aug 8 RgGuinier 3.8 nm Dmax 12.5 nm VolumePorod 151 nm3

SASDH82 – Isoform 3 of Rap guanine nucleotide exchange factor 4, aE2W-DB08 Sample: Isoform 3 of Rap guanine nucleotide exchange factor 4 monomer, 114 kDa Mus musculus protein Buffer: 150 mM NaCl, 1 mM EDTA, 1 mM DTT, and 10 mM Tris-HCl, pH: 7.5 Experiment: SAXS data collected at Anton Paar SAXSess, University of Utah on 2008 Aug 11 RgGuinier 3.6 nm Dmax 11.4 nm VolumePorod 145 nm3