Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy.

Horst BG, Yokom AL, Rosenberg DJ, Morris KL, Hammel M, Hurley JH, Marletta MA
Elife 8 (2019 Sep 30)

PMID: 31566566 doi: 10.7554/eLife.50634

Submitted to SASBDB: 2019 Oct 22 Published in SASBDB: 2019 Dec 17

SASDHA2 – Soluble guanylate cyclase (unbound) Sample: Soluble guanylyl cyclase alpha-1 subunit monomer, 78 kDa Manduca sexta protein Soluble guanylyl cyclase beta-1 subunit monomer, 68 kDa Manduca sexta protein Buffer: 50 mM KH2PO4, 150 mM NaCl, 2% glycerol,, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 3 RgGuinier 4.3 nm Dmax 13.3 nm VolumePorod 230 nm3

SASDHB2 – Soluble guanylate cyclase (nitric oxide, NO, bound) Sample: Soluble guanylyl cyclase alpha-1 subunit monomer, 78 kDa Manduca sexta protein Soluble guanylyl cyclase beta-1 subunit monomer, 68 kDa Manduca sexta protein Buffer: 50 mM KH2PO4,150 mM NaCl, 2% glycerol, 500 µM NO,, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 3 RgGuinier 4.4 nm Dmax 14.2 nm VolumePorod 218 nm3