Therapeutic approaches to ApoE

Lucas Kraft
University of Sussex PhD thesis 2019 (2019 April 25)

URL: https://hdl.handle.net/10779/uos.23467865.v1

Submitted to SASBDB: 2019 Oct 25 Published in SASBDB: 2021 Sep 27

SASDGD7 – Apolipoprotein E3 bound to 330 µg/mL heparin Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 7.3 nm Dmax 25.9 nm

SASDGE7 – Apolipoprotein E3 bound to 110 µg/mL heparin Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 7.5 nm Dmax 25.8 nm

SASDGF7 – Apolipoprotein E3 bound to 37 µg/mL heparin Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.9 nm Dmax 23.9 nm

SASDGG7 – Apolipoprotein E3 bound to 12 µg/mL heparin Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.5 nm Dmax 22.0 nm

SASDGH7 – Apolipoprotein E3 bound to 4 µg/mL heparin Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.3 nm Dmax 20.8 nm

SASDGJ7 – Apolipoprotein E4 bound to 330 µg/mL heparin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 7.6 nm Dmax 26.5 nm

SASDGK7 – Apolipoprotein E4 bound to 110 µg/mL heparin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 7.6 nm Dmax 27.0 nm

SASDGL7 – Apolipoprotein E4 bound to 37 µg/mL heparin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 7.0 nm Dmax 25.8 nm

SASDGM7 – Apolipoprotein E4 bound to 12 µg/mL heparin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.7 nm Dmax 22.0 nm

SASDGN7 – Apolipoprotein E4 bound to 4 µg/mL heparin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.4 nm Dmax 21.2 nm

SASDGP7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 330 µg/mL heparin Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 6.2 nm Dmax 20.8 nm

SASDGQ7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 110 µg/mL heparin Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 6.1 nm Dmax 21.2 nm

SASDGR7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 37 µg/mL heparin Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 6.2 nm Dmax 21.3 nm

SASDGS7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 12 µg/mL heparin Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 6.1 nm Dmax 20.5 nm

SASDGT7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 4 µg/mL heparin Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 6.1 nm Dmax 20.5 nm

SASDGU7 – Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 3.1 nm Dmax 10.5 nm

SASDGV7 – Truncated Apolipoprotein E4 (1-191) bound to 37 µg/mL heparin Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 2.6 nm Dmax 8.2 nm

SASDGW7 – Truncated Apolipoprotein E4 (1-191) bound to 12 µg/mL heparin Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 2.5 nm Dmax 7.8 nm

SASDGX7 – Truncated Apolipoprotein E4 (1-191) bound to 4 µg/mL heparin Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 2.4 nm Dmax 8.0 nm

SASDGY7 – Apolipoprotein E4 (methylated) bound to 330 µg/mL heparin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.9 nm Dmax 23.7 nm

SASDGZ7 – Apolipoprotein E4 (methylated) bound to 110 µg/mL heparin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.8 nm Dmax 23.7 nm

SASDG28 – Apolipoprotein E4 (methylated) bound to 37 µg/mL heparin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.4 nm Dmax 22.2 nm

SASDG38 – Apolipoprotein E4 (methylated) bound to 12 µg/mL heparin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.3 nm Dmax 20.8 nm

SASDG48 – Apolipoprotein E4 (methylated) bound to 4 µg/mL heparin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.1 nm Dmax 20.2 nm

SASDG58 – Apolipoprotein E2 bound to 330 µM Suramin Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.2 nm Dmax 17.8 nm

SASDG68 – Apolipoprotein E2 bound to 110 µM Suramin Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.6 nm Dmax 18.2 nm

SASDG78 – Apolipoprotein E2 bound to 37 µM Suramin Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.9 nm Dmax 19.8 nm

SASDG88 – Apolipoprotein E2 bound to 12 µM Suramin Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.1 nm Dmax 20.3 nm

SASDG98 – Apolipoprotein E2 bound to 4 µM Suramin Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.1 nm Dmax 20.5 nm

SASDGA8 – Apolipoprotein E3 bound to 330 µM Suramin Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.2 nm Dmax 18.6 nm

SASDGB8 – Apolipoprotein E3 bound to 110 µM Suramin Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.6 nm Dmax 19.1 nm

SASDGC8 – Apolipoprotein E3 bound to 37 µM Suramin Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.0 nm Dmax 20.3 nm

SASDGD8 – Apolipoprotein E3 bound to 12 µM Suramin Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.1 nm Dmax 20.0 nm

SASDGE8 – Apolipoprotein E3 bound to 4 µM Suramin Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.2 nm Dmax 20.1 nm

SASDGF8 – Apolipoprotein E4 bound to 330 µM Suramin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.4 nm Dmax 19.6 nm

SASDGG8 – Apolipoprotein E4 bound to 110 µM Suramin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.5 nm Dmax 19.5 nm

SASDGH8 – Apolipoprotein E4 bound to 37 µM Suramin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.0 nm Dmax 20.1 nm

SASDGJ8 – Apolipoprotein E4 bound to 12 µM Suramin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.1 nm Dmax 20.1 nm

SASDGK8 – Apolipoprotein E4 bound to 4 µM Suramin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.2 nm Dmax 20.4 nm

SASDGL8 – Apolipoprotein E4 (K143A K146A) mutant bound to 330 µM Suramin Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 6.0 nm Dmax 22.4 nm

SASDGM8 – Apolipoprotein E4 (K143A K146A) mutant bound to 110 µM Suramin Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 5.5 nm Dmax 18.9 nm

SASDGN8 – Apolipoprotein E4 (K143A K146A) mutant bound to 37 µM Suramin Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 5.9 nm Dmax 19.9 nm

SASDGP8 – Apolipoprotein E4 (K143A K146A) mutant bound to12 µM Suramin Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 6.0 nm Dmax 20.2 nm

SASDGQ8 – Apolipoprotein E4 (K143A K146A) mutant bound to 4 µM Suramin Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 6.1 nm Dmax 20.4 nm

SASDGR8 – Apolipoprotein E4 (methylated) bound to 330 µM Suramin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.0 nm Dmax 20.6 nm

SASDGS8 – Apolipoprotein E4 (methylated) bound to 110 µM Suramin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.7 nm Dmax 18.1 nm

SASDGT8 – Apolipoprotein E4 (methylated) bound to 37 µM Suramin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.8 nm Dmax 19.6 nm

SASDGU8 – Apolipoprotein E4 (methylated) bound to 12 µM Suramin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.0 nm Dmax 19.4 nm

SASDFV8 – Apolipoprotein E2 (ApoE2) bound to 4 µg/mL heparin Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.2 nm Dmax 20.7 nm VolumePorod 504 nm3

SASDGV8 – Apolipoprotein E4 (methylated) bound to 4 µM Suramin Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.0 nm Dmax 19.7 nm

SASDFW8 – Apolipoprotein E2 (ApoE2) bound to 12 µg/mL heparin Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.4 nm Dmax 21.7 nm VolumePorod 531 nm3

SASDGW8 – Truncated Apolipoprotein E4 (1-191) bound to 330 µM Suramin Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 3.1 nm Dmax 11.1 nm

SASDFX8 – Apolipoprotein E2 (ApoE2) bound to 37 µg/mL heparin Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.5 nm Dmax 23.4 nm VolumePorod 584 nm3

SASDGX8 – Truncated Apolipoprotein E4 (1-191) bound to 110 µM Suramin Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 2.8 nm Dmax 9.5 nm

SASDFY8 – Apolipoprotein E2 (ApoE2) bound to 110 µg/mL heparin Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 7.1 nm Dmax 24.0 nm VolumePorod 666 nm3

SASDGY8 – Truncated Apolipoprotein E4 (1-191) bound to 37 µM Suramin Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 2.6 nm Dmax 8.0 nm

SASDFZ8 – Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 7.2 nm Dmax 24.5 nm VolumePorod 696 nm3

SASDGZ8 – Truncated Apolipoprotein E4 (1-191) bound to 12 µM Suramin Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 2.5 nm Dmax 8.2 nm

SASDG29 – Truncated Apolipoprotein E4 (1-191) bound to 4 µM Suramin Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 2.4 nm Dmax 7.8 nm

SASDG39 – Apolipoprotein E2 Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.2 nm Dmax 20.5 nm

SASDG49 – Apolipoprotein E3 Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.1 nm Dmax 20.6 nm

SASDG59 – Apolipoprotein E4 Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.2 nm Dmax 20.8 nm

SASDG69 – Apolipoprotein E4 (K143A K146A) mutant Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 6.2 nm Dmax 20.7 nm

SASDG79 – Apolipoprotein E4 (methylated) Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.1 nm Dmax 20.3 nm

SASDG89 – Truncated Apolipoprotein E4 (1-191) Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 2.4 nm Dmax 7.7 nm

SASDG99 – Apolipoprotein E2 (SEC-SAXS) Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.8 nm Dmax 20.0 nm

SASDGA9 – Apolipoprotein E2 bound to 1 mg/mL heparin (SEC-SAXS) Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.1 nm Dmax 21.0 nm

SASDGB9 – Apolipoprotein E2 bound to 1 mM Suramin (SEC-SAXS) Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.1 nm Dmax 18.3 nm

SASDGC9 – Apolipoprotein E3 (SEC-SAXS) Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.8 nm Dmax 20.0 nm

SASDGD9 – Apolipoprotein E3 bound to 1 mg/mL heparin (SEC-SAXS) Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.1 nm Dmax 21.3 nm

SASDGE9 – Apolipoprotein E3 bound to 1 mM Suramin (SEC-SAXS) Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.1 nm Dmax 19.3 nm

SASDGF9 – Apolipoprotein E4 (SEC-SAXS) Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.9 nm Dmax 20.0 nm

SASDGG9 – Apolipoprotein E4 bound to 1 mg/mL heparin (SEC-SAXS) Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.2 nm Dmax 21.6 nm

SASDGH9 – Apolipoprotein E4 bound to 1 mM Suramin (SEC-SAXS) Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.6 nm Dmax 18.7 nm

SASDGJ9 – Apolipoprotein E4 (methylated) - (SEC-SAXS) Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.9 nm Dmax 20.0 nm

SASDGK9 – Apolipoprotein E4 (methylated) bound to 1 mg/mL heparin (SEC-SAXS) Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 6.4 nm Dmax 21.7 nm

SASDGL9 – Apolipoprotein E4 (methylated) bound to 1 mM Suramin (SEC-SAXS) Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 5.3 nm Dmax 18.5 nm

SASDGM9 – Mutant Apolipoprotein E4 (K143A K146A) - (SEC-SAXS) Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 5.8 nm Dmax 20.2 nm

SASDGN9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mg/mL heparin (SEC-SAXS) Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 5.7 nm Dmax 19.8 nm

SASDGP9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mM Suramin (SEC-SAXS) Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21 RgGuinier 5.4 nm Dmax 18.3 nm

SASDGQ9 – Truncated Apolipoprotein E4 (1-191) - (SEC-SAXS) Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 2.3 nm Dmax 7.6 nm

SASDGR9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC-SAXS) Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 4.2 nm Dmax 16.8 nm

SASDGS9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS) Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 2.4 nm Dmax 8.3 nm

SASDGT9 – Truncated Apolipoprotein E4 (1-191) bound to 110 µg/mL heparin Sample: Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3 RgGuinier 2.7 nm Dmax 8.3 nm