Inhibition, crystal structures, and in-solution oligomeric structure of aldehyde dehydrogenase 9A1.

Wyatt JW, Korasick DA, Qureshi IA, Campbell AC, Gates KS, Tanner JJ
Arch Biochem Biophys :108477 (2020 Jul 24)
PMID: 32717224
doi: 10.1016/j.abb.2020.108477 		Submitted to SASBDB: 2020 Feb 10
Published in SASBDB:

SASDHV5 – Human 4-trimethylaminobutyraldehyde dehydrogenase (ALDH9A1) at 1.25 mg/mL

4-trimethylaminobutyraldehyde dehydrogenase experimental SAS data
OTHER model
Sample: 4-trimethylaminobutyraldehyde dehydrogenase tetramer, 215 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 600 mM NaCl, 2% (v/v) glycerol, 1 mM DTT, 1 mM NAD+, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 28
RgGuinier 3.8 nm
Dmax 10.9 nm
VolumePorod 252 nm3

SASDHW5 – Human 4-trimethylaminobutyraldehyde dehydrogenase (ALDH9A1) at 2.5 mg/mL

4-trimethylaminobutyraldehyde dehydrogenase experimental SAS data
OTHER model
Sample: 4-trimethylaminobutyraldehyde dehydrogenase tetramer, 215 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 600 mM NaCl, 2% (v/v) glycerol, 1 mM DTT, 1 mM NAD+, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 28
RgGuinier 3.8 nm
Dmax 10.8 nm
VolumePorod 240 nm3

SASDHX5 – Human 4-trimethylaminobutyraldehyde dehydrogenase (ALDH9A1) at 5 mg/mL

4-trimethylaminobutyraldehyde dehydrogenase experimental SAS data
OTHER model
Sample: 4-trimethylaminobutyraldehyde dehydrogenase tetramer, 215 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 600 mM NaCl, 2% (v/v) glycerol, 1 mM DTT, 1 mM NAD+, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 28
RgGuinier 3.8 nm
Dmax 10.5 nm
VolumePorod 240 nm3