KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.

Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE
Nucleic Acids Res 43(20):10039-54 (2015 Nov 16)

PMID: 26424851 doi: 10.1093/nar/gkv987

Submitted to SASBDB: 2015 Jul 6 Published in SASBDB: 2016 Mar 17

SASDAM8 – MHV-68 LANA Sample: Latency-associated nuclear antigen tetramer, 87 kDa Murid herpesvirus 4 protein Buffer: 25 mM Na/K Phosphate, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Apr 27 RgGuinier 4.2 nm Dmax 16.0 nm VolumePorod 117 nm3

SASDAN8 – mLBS1-2 DNA Sample: MHV-68 TR DNA monomer, 30 kDa unidentified herpesvirus DNA Buffer: 10 mM TRIS 150 mM NaCl, pH: 7.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Apr 27 RgGuinier 4.0 nm Dmax 16.0 nm VolumePorod 50 nm3

SASDAP8 – kLBS1-2 DNA Sample: KLBS1-2 DNA monomer, 24 kDa unidentified herpesvirus DNA Buffer: Tris, pH: 7.6 Experiment: SAXS data collected at BM29, ESRF on 2013 Apr 27 RgGuinier 4.0 nm Dmax 16.0 nm VolumePorod 50 nm3

SASDAQ8 – kLANA mutant dimer-tetramer mixture Sample: ORF73 tetramer tetramer, 63 kDa Human herpesvirus 8 protein ORF73 dimer dimer, 32 kDa Human herpesvirus 8 protein Buffer: 25 mM Na/K Phosphate, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2014 Jun 21 RgGuinier 2.4 nm Dmax 9.5 nm VolumePorod 50 nm3

SASDAR8 – mLANA 124-316 mLBS1-2 8:1 complex Sample: MHV-68 TR DNA monomer, 30 kDa unidentified herpesvirus DNA Latency-associated nuclear antigen octamer, 269 kDa Murid herpesvirus 4 protein Buffer: 25 mM Na/K Phosphate, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Apr 27 RgGuinier 5.8 nm Dmax 20.0 nm VolumePorod 475 nm3

SASDAS8 – kLANA 1008-1150 -- kLBS1-2 complex 8:2 (partially dissociated) Sample: KLBS1-2 DNA monomer, 24 kDa unidentified herpesvirus DNA ORF73 tetramer tetramer, 63 kDa Human herpesvirus 8 protein ORF73 octamer octamer, 126 kDa Human herpesvirus 8 protein KLBS1-2 DNA two monomers dimer, 48 kDa unidentified herpesvirus RNA Buffer: 25 mM Na/K Phosphate, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Apr 27 RgGuinier 4.8 nm Dmax 16.0 nm VolumePorod 250 nm3