The hTERT core promoter forms three parallel G-quadruplexes.

Monsen RC, DeLeeuw L, Dean WL, Gray RD, Sabo TM, Chakravarthy S, Chaires JB, Trent JO
Nucleic Acids Res (2020 Feb 21)

PMID: 32083666 doi: 10.1093/nar/gkaa107

Submitted to SASBDB: 2019 Nov 19 Published in SASBDB: 2020 Feb 25

SASDHM3 – Human Telomerase Reverse Transcriptase Core Promoter G-rich Region (hTERT_WT) DNA Sample: Human Telomerase Reverse Transcriptase Core Promoter G-rich Region monomer, 22 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Oct 5 RgGuinier 2.2 nm Dmax 8.1 nm VolumePorod 28 nm3

SASDHN3 – Antiparallel Hairpin (AH) DNA Sample: Antiparallel Hairpin monomer, 21 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 3 RgGuinier 2.5 nm Dmax 8.1 nm VolumePorod 28 nm3

SASDHP3 – Optimized Parallel DNA Sample: Optimized Parallel monomer, 18 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 3 RgGuinier 1.7 nm Dmax 5.0 nm VolumePorod 17 nm3

SASDHQ3 – Human Telomerase Reverse Transcriptase Core Promoter (hTERT) PQS2-PQS3 region, WT_PQS23 Sample: HTERT core promoter PQS2-PQS3 region monomer, 14 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 3 RgGuinier 1.7 nm Dmax 5.4 nm VolumePorod 18 nm3

SASDHR3 – Antiparallel Hairpin PQS2-PQS3 segment DNA, AH_PQS23 Sample: Antiparallel Hairpin PQS2-PQS3 segment monomer, 14 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 3 RgGuinier 2.0 nm Dmax 6.4 nm VolumePorod 18 nm3