Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination.

Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M
Angew Chem Int Ed Engl (2020 Dec 15)

PMID: 33320993 doi: 10.1002/anie.202011548

Submitted to SASBDB: 2020 Jan 10 Published in SASBDB: 2020 Dec 20

SASDHU4 – ECSIT C-terminal domain Sample: Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial dimer, 44 kDa Homo sapiens protein Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 7 RgGuinier 3.7 nm Dmax 11.9 nm VolumePorod 117 nm3

SASDHV4 – Mitochondrial complex I assembly factor, NDUFAF1 Sample: Complex I intermediate-associated protein 30, mitochondrial monomer, 35 kDa Homo sapiens protein Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 May 29 RgGuinier 3.3 nm Dmax 9.6 nm VolumePorod 82 nm3

SASDHW4 – Mitochondrial Complex I assembly factor, ACAD9 Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 132 kDa Homo sapiens protein Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 May 28 RgGuinier 3.6 nm Dmax 10.2 nm VolumePorod 217 nm3

SASDHX4 – Mitochondrial complex I assembly factor, ACAD9, chimeric construct where segment 445-482 has been replaced with segment 481-518 of VLCAD protein Sample: Complex I assembly factor ACAD9-VLCAD chimera dimer, 131 kDa Homo sapiens protein Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 30 RgGuinier 3.9 nm Dmax 11.7 nm VolumePorod 217 nm3

SASDHY4 – Mitochondrial very long-chain specific acyl-CoA dehydrogenase enzyme, VLCAD Sample: Very long-chain specific acyl-CoA dehydrogenase, mitochondrial dimer, 126 kDa Homo sapiens protein Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 2 RgGuinier 3.4 nm Dmax 9.9 nm VolumePorod 215 nm3

SASDHZ4 – The C-terminal domain of ESCIT bound to a dimer of the complex I assembly factor ACAD9 Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 132 kDa Homo sapiens protein Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial tetramer, 88 kDa Homo sapiens protein Buffer: 25 mM HEPES, 250 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 4 RgGuinier 6.0 nm Dmax 16.5 nm VolumePorod 683 nm3