An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA.

Baughman HER, Narang D, Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
J Biol Chem :102349 (2022 Aug 4)

PMID: 35934050 doi: 10.1016/j.jbc.2022.102349

Submitted to SASBDB: 2020 Jan 20 Published in SASBDB: 2022 Aug 9

SASDHB5 – RelA Homology Domain of p50/RelA heterodimer Sample: NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein Transcription factor p65 19-321 monomer, 35 kDa Mus musculus protein Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11 RgGuinier 3.8 nm Dmax 13.0 nm VolumePorod 110 nm3

SASDHC5 – Nuclear factor kB p50/RelA heterodimer Sample: Transcription factor p65 19-549 monomer, 58 kDa Mus musculus protein NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11 RgGuinier 4.6 nm Dmax 15.3 nm VolumePorod 183 nm3

SASDHD5 – Transactivation domain of RelA Sample: Transcription factor p65 340-549 monomer, 23 kDa Mus musculus protein Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11 RgGuinier 2.7 nm Dmax 8.2 nm VolumePorod 59 nm3

SASDHE5 – Rel Homology Domain of p50/RelA bound to IFN kB site DNA Sample: NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein Transcription factor p65 19-321 monomer, 35 kDa Mus musculus protein IFN kB DNA monomer, 8 kDa DNA Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11 RgGuinier 4.3 nm Dmax 13.0 nm VolumePorod 237 nm3

SASDHF5 – Rel Homology Domain of p50/RelA bound to Urokinase kB site DNA Sample: NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein Transcription factor p65 19-321 monomer, 35 kDa Mus musculus protein Urokinase kB monomer, 8 kDa DNA Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11 RgGuinier 4.1 nm Dmax 13.0 nm VolumePorod 244 nm3

SASDHG5 – Nuclear factor kB p50/RelA bound to IFN kB site DNA Sample: Transcription factor p65 19-549 monomer, 58 kDa Mus musculus protein NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein IFN kB DNA monomer, 8 kDa DNA Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 4 RgGuinier 5.1 nm Dmax 18.9 nm VolumePorod 314 nm3

SASDHH5 – Nuclear factor kB p50/RelA bound to Urokinase kB site DNA Sample: Transcription factor p65 19-549 monomer, 58 kDa Mus musculus protein NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein Urokinase kB monomer, 8 kDa DNA Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 4 RgGuinier 4.7 nm Dmax 17.5 nm VolumePorod 201 nm3