c-di-AMP hydrolysis by a novel type of phosphodiesterase promotes differentiation of multicellular bacteria

Latoscha A, Drexler D, Al-Bassam M, Kaever V, Findlay K, Witte G, Tschowri N
(2019 Oct 01)

doi: 10.1101/789354 		Submitted to SASBDB: 2020 Jan 16
Published in SASBDB:

SASDH25 – Actinobacterial phosphodiesterase targeting cyclic di-AMP (AtaC)

Alkaline phosphodiesterase I or Nucleotide pyrophosphatase experimental SAS data
Alkaline phosphodiesterase I or Nucleotide pyrophosphatase Kratky plot
Sample: Alkaline phosphodiesterase I or Nucleotide pyrophosphatase monomer, 46 kDa Streptomyces venezuelae protein
Buffer: 20 mM HEPES, 200mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 17
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 74 nm3

SASDH35 – Soluble cyclic di-AMP binding protein CpeA (vnz_28055), regulator of CpeB

Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) experimental SAS data
Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) Kratky plot
Sample: Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) dimer, 38 kDa Streptomyces venezuelae protein
Buffer: 200 mM NaCl, 30 mM NaPi, 5% (v/v) glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 4
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 69 nm3