Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP

Dominguez-Martin M, Hammel M, Gupta S, Lechno-Yossef S, Sutter M, Rosenberg D, Chen Y, Petzold C, Ralston C, Polívka T, Kerfeld C
Scientific Reports 10(1) (2020 Sep 23)

doi: 10.1038/s41598-020-72383-y

Submitted to SASBDB: 2020 Feb 17 Published in SASBDB: 2020 Sep 29

SASDHC6 – C-terminal domain-like carotenoid protein (CCP2), apo-dimer Sample: C-terminal domain-like carotenoid protein dimer, 33 kDa Tolypothrix sp. PCC … protein Buffer: 10 mM potassium phosphate, 100 mM NaCl, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15 RgGuinier 2.5 nm Dmax 9.1 nm VolumePorod 55 nm3

SASDHD6 – C-terminal domain-like carotenoid protein (CCP2), holo-dimer Sample: C-terminal domain-like carotenoid protein dimer, 36 kDa Tolypothrix sp. PCC … protein Buffer: 10 mM potassium phosphate, 100 mM NaCl, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15 RgGuinier 2.5 nm Dmax 7.9 nm VolumePorod 62 nm3

SASDHE6 – C-terminal domain-like carotenoid protein (CCP2), apo-tetramer Sample: C-terminal domain-like carotenoid protein tetramer, 66 kDa Tolypothrix sp. PCC … protein Buffer: 10 mM potassium phosphate, 100 mM NaCl, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15 RgGuinier 3.2 nm Dmax 10.0 nm VolumePorod 110 nm3

SASDHF6 – C-terminal domain-like carotenoid protein (CCP2), holo-tetramer Sample: C-terminal domain-like carotenoid protein tetramer, 73 kDa Tolypothrix sp. PCC … protein Buffer: 10 mM potassium phosphate, 100 mM NaCl, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15 RgGuinier 3.3 nm Dmax 12.1 nm VolumePorod 157 nm3