Solution structure of Plasmodium falciparum Hsp90 indicates a high flexible dimer

Silva N, Torricillas M, Minari K, Barbosa L, Seraphim T, Borges J
Archives of Biochemistry and Biophysics :108468 (2020 Jul)

doi: 10.1016/j.abb.2020.108468 		Submitted to SASBDB: 2020 Mar 25
Published in SASBDB:

SASDHD9 – Full-length Plasmodium falciparum Heat shock protein 90 (PfHsp90)

Plasmodium falciparum Heat shock protein 90 experimental SAS data
DAMMIN model
Sample: Plasmodium falciparum Heat shock protein 90 dimer, 177 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM KCl, 1 mM β-mercaptoethanol, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 11
RgGuinier 5.7 nm
Dmax 19.0 nm
VolumePorod 350 nm3

SASDHE9 – Plasmodium falciparum Heat shock protein 90 (PfHsp90) N- and M-domains

Plasmodium falciparum Heat shock protein 90 N-terminal and Middle domains experimental SAS data
Plasmodium falciparum Heat shock protein 90 (PfHsp90) N- and M-domains Rg histogram
Sample: Plasmodium falciparum Heat shock protein 90 N-terminal and Middle domains monomer, 68 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM KCl, 1 mM β-mercaptoethanol, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 11
RgGuinier 3.9 nm
Dmax 14.0 nm
VolumePorod 106 nm3

SASDHF9 – Plasmodium falciparum Heat shock protein 90 (PfHsp90) M-domain

Plasmodium falciparum Heat shock protein 90 middle domain experimental SAS data
DAMMIN model
Sample: Plasmodium falciparum Heat shock protein 90 middle domain monomer, 33 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM KCl, 1 mM β-mercaptoethanol, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 11
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 47 nm3