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44 hits found for Al Kikhney

SASDJ92 – S. epidermidis extracellular matrix binding protein (Embp) F-repeats

Extracellular matrix binding protein F-repeats experimental SAS data
SASREF model
Sample: Extracellular matrix binding protein F-repeats , 70 kDa Staphylococcus epidermidis protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 15
A Giant Extracellular Matrix Binding Protein of Staphylococcus epidermidis Binds Surface-Immobilized Fibronectin via a Novel Mechanism. mBio 11(5) (2020)
...Kikhney A, Wolters M, Christner M, Heise M, Wilde J, Weißelberg S, Both A, Betzel C, Hammerschmidt S, Svergun D, Aepfelbacher M, Rohde H
RgGuinier 7.0 nm
Dmax 28.0 nm

SASDJA2 – S. epidermidis extracellular matrix binding protein (Embp) FG-repeats

Extracellular matrix binding protein FG-repeats experimental SAS data
EOM/RANCH model
Sample: Extracellular matrix binding protein FG-repeats , 85 kDa Staphylococcus epidermidis protein
Buffer: PBS, 136.5 mM NaCl, 2.65 mM KCl, 8.3 mM Na2HPO4, 2.65 mM KH2PO4, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 14
A Giant Extracellular Matrix Binding Protein of Staphylococcus epidermidis Binds Surface-Immobilized Fibronectin via a Novel Mechanism. mBio 11(5) (2020)
...Kikhney A, Wolters M, Christner M, Heise M, Wilde J, Weißelberg S, Both A, Betzel C, Hammerschmidt S, Svergun D, Aepfelbacher M, Rohde H
RgGuinier 10.7 nm
Dmax 40.0 nm

SASDPG2 – Spiegelmer NOX-E36, 1.15 mg/ml

Spiegelmer NOX-E36 experimental SAS data
MC-FOLD | MC-SYM model
Sample: Spiegelmer NOX-E36 monomer, 13 kDa synthetic construct RNA
Buffer: 20 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 20
Spiegelmers® NOX-E36 and NOX-A12
Al Kikhney, Bara Schmidt
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 18 nm3

SASDPH2 – Spiegelmer NOX-E36, 4.46 mg/ml

Spiegelmer NOX-E36 experimental SAS data
MC-FOLD | MC-SYM model
Sample: Spiegelmer NOX-E36 monomer, 13 kDa synthetic construct RNA
Buffer: 20 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 20
Spiegelmers® NOX-E36 and NOX-A12
Al Kikhney, Bara Schmidt
RgGuinier 2.1 nm

SASDPJ2 – Spiegelmer NOX-A12, 1.0 mg/ml

Spiegelmer NOX-A12 experimental SAS data
MC-FOLD | MC-SYM model
Sample: Spiegelmer NOX-A12 monomer, 15 kDa synthetic construct RNA
Buffer: 20 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 20
Spiegelmers® NOX-E36 and NOX-A12
Al Kikhney, Bara Schmidt
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 21 nm3

SASDPK2 – Spiegelmer NOX-A12, 3.5 mg/ml

Spiegelmer NOX-A12 experimental SAS data
MC-FOLD | MC-SYM model
Sample: Spiegelmer NOX-A12 monomer, 15 kDa synthetic construct RNA
Buffer: 20 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 20
Spiegelmers® NOX-E36 and NOX-A12
Al Kikhney, Bara Schmidt
RgGuinier 2.0 nm
Dmax 7.6 nm
VolumePorod 18 nm3

SASDAR3 – ...al binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein experimental SAS data
PsrP functional binding region Rg histogram
Sample: ...al binding region (187-385) of the pneumococcal serine-rich repeat protein monomer, 22 kDa Streptococcus pneumoniae protein
Buffer: 20 mM sodium citrate 250 mM NaCl 2.5 % Glycerol, pH: 5.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2
...al serine-rich protein PsrP adopts a novel MSCRAMM fold. Open Biol 4:130090 (2014)
...Kikhney A, Hentrich K, Diamante A, Ebel C, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 2.3 nm
Dmax 7.7 nm
VolumePorod 37 nm3

SASDBF4 – Wild-type LytA (N-His6) with choline

Lytic Amidase with choline experimental SAS data
SASREF model
Sample: Lytic Amidase with choline dimer, 75 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2
...al and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
...alova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 4.9 nm
Dmax 15.0 nm
VolumePorod 115 nm3

SASDBG4 – Prophage LytA with choline

Prophage Lytic Amidase with choline experimental SAS data
SASREF model
Sample: Prophage Lytic Amidase with choline dimer, 73 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2
...al and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
...alova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 5.9 nm
Dmax 17.0 nm
VolumePorod 130 nm3

SASDBH4 – Wild-type LytA (N-His6) without choline

Lytic Amidase without choline experimental SAS data
SASREF model
Sample: Lytic Amidase without choline monomer, 37 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 3
...al and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
...alova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 3.4 nm
Dmax 12.5 nm
VolumePorod 56 nm3

SASDBJ4 – Wild-type LytA choline-binding domain

Lytic Amidase choline-binding domain experimental SAS data
SASREF model
Sample: Lytic Amidase choline-binding domain dimer, 17 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 11
...al and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
...alova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 3.3 nm
Dmax 10.0 nm
VolumePorod 49 nm3

SASDBN4 – ...allose synthase

Callose synthase experimental SAS data
CORAL model
Sample: ...allose synthase octamer, 633 kDa ...aliana protein
Buffer: Tris, 50 mM NaCl, pH: 7.3
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
...al Characterization of Cell Wall and Plasma Membrane Proteins of Arabidopsis thaliana University of Hamburg Dissertation 8022 (2016)
Haifa El Kilani
RgGuinier 8.0 nm
Dmax 30.0 nm
VolumePorod 1033 nm3

SASDB35 – Staphylococcus aureus thiaminase II

Thiaminase type II enzyme experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Thiaminase type II enzyme tetramer, 107 kDa Staphylococcus aureus protein
Buffer: 100 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 May 11
Staphylococcus aureus thiaminase II: oligomerization warrants proteolytic protection against serine proteases. ...allogr D Biol Crystallogr 69(Pt 12):2320-9 (2013)
...Kikhney A, Müller IB, Perbandt M, Svergun D, Wrenger C, Betzel C
RgGuinier 3.4 nm
Dmax 11.0 nm
VolumePorod 168 nm3

SASDC56 – Blue light receptor YtvA, dark/inactive state

pfyP - Blue light photoreceptor experimental SAS data
GASBOR model
Sample: pfyP - Blue light photoreceptor dimer, 58 kDa Bacillus subtilis protein
Buffer: PBS + 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Oct 24
...aled by a combined AUC and SAXS study. J Mol Biol 403(1):78-87 (2010)
...Kikhney A, Svergun D, Gärtner W, Schmieder P
RgGuinier 3.2 nm
Dmax 10.1 nm
VolumePorod 75 nm3

SASDC66 – Blue light receptor YtvA, lit/active state

pfyP - Blue light photoreceptor experimental SAS data
GASBOR model
Sample: pfyP - Blue light photoreceptor dimer, 58 kDa Bacillus subtilis protein
Buffer: PBS + 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Oct 24
...aled by a combined AUC and SAXS study. J Mol Biol 403(1):78-87 (2010)
...Kikhney A, Svergun D, Gärtner W, Schmieder P
RgGuinier 3.2 nm
Dmax 10.1 nm
VolumePorod 74 nm3

SASDB86 – apo-BRD4

Bromodomain-containing protein 4 experimental SAS data
DAMMIF model
Sample: Bromodomain-containing protein 4 monomer, 56 kDa Homo sapiens protein
Buffer: 20mM Hepes, 100mM NaCl, 1mM Tris(2-carboxyethyl)phosphine hydrochloride, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 12
...alent inhibitors of BET bromodomains. Nat Chem Biol 12(12):1097-1104 (2016)
...alker G, Bobby R, Boiko S, Bradbury RH, Callis R, Clark E, Dale I, Daniels DL, Dulak A, Flavell L, Holdgate G, Jowitt TA, Kikhney A, McAlister M, Méndez J, Ogg D, Patel J, Petteruti P, Robb GR, Robers...
RgGuinier 7.0 nm
Dmax 27.0 nm

SASDB96 – Compound 1:BRD4 (1:1)

Bromodomain-containing protein 4 experimental SAS data
DAMMIF model
Sample: Bromodomain-containing protein 4 monomer, 56 kDa Homo sapiens protein
Buffer: 20mM Hepes, 100mM NaCl, 1mM Tris(2-carboxyethyl)phosphine hydrochloride, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 12
...alent inhibitors of BET bromodomains. Nat Chem Biol 12(12):1097-1104 (2016)
...alker G, Bobby R, Boiko S, Bradbury RH, Callis R, Clark E, Dale I, Daniels DL, Dulak A, Flavell L, Holdgate G, Jowitt TA, Kikhney A, McAlister M, Méndez J, Ogg D, Patel J, Petteruti P, Robb GR, Robers...
RgGuinier 6.2 nm
Dmax 27.5 nm

SASDBA6 – Compound 1:BRD4 (2:1)

Bromodomain-containing protein 4 experimental SAS data
DAMMIF model
Sample: Bromodomain-containing protein 4 monomer, 56 kDa Homo sapiens protein
Buffer: 20mM Hepes, 100mM NaCl, 1mM Tris(2-carboxyethyl)phosphine hydrochloride, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 12
...alent inhibitors of BET bromodomains. Nat Chem Biol 12(12):1097-1104 (2016)
...alker G, Bobby R, Boiko S, Bradbury RH, Callis R, Clark E, Dale I, Daniels DL, Dulak A, Flavell L, Holdgate G, Jowitt TA, Kikhney A, McAlister M, Méndez J, Ogg D, Patel J, Petteruti P, Robb GR, Robers...
RgGuinier 6.3 nm
Dmax 24.5 nm

SASDBB6 – Compound 6:BRD4 (1:1)

Bromodomain-containing protein 4 experimental SAS data
DAMMIF model
Sample: Bromodomain-containing protein 4 monomer, 56 kDa Homo sapiens protein
Buffer: 20mM Hepes, 100mM NaCl, 1mM Tris(2-carboxyethyl)phosphine hydrochloride, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 8
...alent inhibitors of BET bromodomains. Nat Chem Biol 12(12):1097-1104 (2016)
...alker G, Bobby R, Boiko S, Bradbury RH, Callis R, Clark E, Dale I, Daniels DL, Dulak A, Flavell L, Holdgate G, Jowitt TA, Kikhney A, McAlister M, Méndez J, Ogg D, Patel J, Petteruti P, Robb GR, Robers...
RgGuinier 5.1 nm
Dmax 24.3 nm

SASDBC6 – Compound 6:BRD4 (2:1)

Bromodomain-containing protein 4 experimental SAS data
DAMMIF model
Sample: Bromodomain-containing protein 4 monomer, 56 kDa Homo sapiens protein
Buffer: 20mM Hepes, 100mM NaCl, 1mM Tris(2-carboxyethyl)phosphine hydrochloride, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 8
...alent inhibitors of BET bromodomains. Nat Chem Biol 12(12):1097-1104 (2016)
...alker G, Bobby R, Boiko S, Bradbury RH, Callis R, Clark E, Dale I, Daniels DL, Dulak A, Flavell L, Holdgate G, Jowitt TA, Kikhney A, McAlister M, Méndez J, Ogg D, Patel J, Petteruti P, Robb GR, Robers...
RgGuinier 4.4 nm
Dmax 17.3 nm

SASDAF7 – ...al-bound hETHE1

Persulfide dioxygenase ETHE1, mitochondrial experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...al dimer, 56 kDa Homo sapiens protein
Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Oct 21
...al significance of the Homo sapiens ethylmalonic encephalopathy protein iron binding site
Al Kikhney, Marco ...alomone-Stagni
RgGuinier 2.5 nm
Dmax 7.5 nm
VolumePorod 70 nm3

SASDAH7 – ...al-free hETHE1, 0.5 mg/ml

Persulfide dioxygenase ETHE1, mitochondrial experimental SAS data
DAMMIN model
Sample: ...al dimer, 56 kDa Homo sapiens protein
Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23
...al significance of the Homo sapiens ethylmalonic encephalopathy protein iron binding site
Al Kikhney, Marco ...alomone-Stagni
RgGuinier 9.0 nm
Dmax 32.3 nm
VolumePorod 400 nm3

SASDAJ7 – ...al-free hETHE1, 1 mg/ml

Persulfide dioxygenase ETHE1, mitochondrial experimental SAS data
DAMMIN model
Sample: ...al dimer, 56 kDa Homo sapiens protein
Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23
...al significance of the Homo sapiens ethylmalonic encephalopathy protein iron binding site
Al Kikhney, Marco ...alomone-Stagni
RgGuinier 8.3 nm
Dmax 38.0 nm
VolumePorod 602 nm3

SASDAK7 – ...al-free hETHE1, 2 mg/ml

Persulfide dioxygenase ETHE1, mitochondrial experimental SAS data
DAMMIN model
Sample: ...al dimer, 56 kDa Homo sapiens protein
Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23
...al significance of the Homo sapiens ethylmalonic encephalopathy protein iron binding site
Al Kikhney, Marco ...alomone-Stagni
RgGuinier 8.8 nm
Dmax 42.5 nm
VolumePorod 647 nm3

...AL7 – ...al-free hETHE1, 3 mg/ml

Persulfide dioxygenase ETHE1, mitochondrial experimental SAS data
DAMMIN model
Sample: ...al dimer, 56 kDa Homo sapiens protein
Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23
...al significance of the Homo sapiens ethylmalonic encephalopathy protein iron binding site
Al Kikhney, Marco ...alomone-Stagni
RgGuinier 11.2 nm
Dmax 56.0 nm
VolumePorod 1445 nm3

SASDAM7 – ...al-free hETHE1, 5 mg/ml

Persulfide dioxygenase ETHE1, mitochondrial experimental SAS data
DAMMIN model
Sample: ...al dimer, 56 kDa Homo sapiens protein
Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23
...al significance of the Homo sapiens ethylmalonic encephalopathy protein iron binding site
Al Kikhney, Marco ...alomone-Stagni
RgGuinier 12.4 nm
Dmax 59.0 nm
VolumePorod 1820 nm3

SASDAN7 – ...al-free hETHE1, 10 mg/ml

Persulfide dioxygenase ETHE1, mitochondrial experimental SAS data
DAMMIN model
Sample: ...al dimer, 56 kDa Homo sapiens protein
Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23
...al significance of the Homo sapiens ethylmalonic encephalopathy protein iron binding site
Al Kikhney, Marco ...alomone-Stagni
RgGuinier 14.2 nm
Dmax 63.5 nm
VolumePorod 2317 nm3

SASDAU8 – Immunosuppressive virulence protein YopM

Yersinia outer protein M (34-481) experimental SAS data
SASREF model
Sample: Yersinia outer protein M (34-481) dimer, 101 kDa Yersinia enterocolitica protein
Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 13
...al S6 Kinase in the Nucleus of Host Cells. PLoS Pathog 12(6):e1005660 (2016)
...Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M
RgGuinier 3.9 nm
Dmax 11.5 nm
VolumePorod 154 nm3

SASDAV8 – DEAD box helicase DDX3

DEAD box RNA helicase DDX3 (51-418) experimental SAS data
SASREF model
Sample: DEAD box RNA helicase DDX3 (51-418) monomer, 42 kDa Yersinia enterocolitica protein
Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 13
...al S6 Kinase in the Nucleus of Host Cells. PLoS Pathog 12(6):e1005660 (2016)
...Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M
RgGuinier 2.8 nm
Dmax 12.0 nm
VolumePorod 80 nm3

SASDAW8 – 2:1 YopM:DDX3 complex

Yersinia outer protein M (34-481)DEAD box RNA helicase DDX3 (51-418) experimental SAS data
SASREF model
Sample: Yersinia outer protein M (34-481) dimer, 101 kDa Yersinia enterocolitica protein
DEAD box RNA helicase DDX3 (51-418) monomer, 42 kDa Yersinia enterocolitica protein
Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 14
...al S6 Kinase in the Nucleus of Host Cells. PLoS Pathog 12(6):e1005660 (2016)
...Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M
RgGuinier 4.0 nm
Dmax 15.0 nm
VolumePorod 190 nm3

SASDAX8 – Ribokinase ThiM

Ribokinase ThiM experimental SAS data
CORAL model
Sample: Ribokinase ThiM trimer, 89 kDa Staphylococcus aureus protein
Buffer: 50 mM Potassium phosphate 10 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Nov 19
Structure of ThiM from Vitamin B1 biosynthetic pathway of Staphylococcus aureus - Insights into a novel pro-drug approach addressing MRSA infections. Sci Rep 6:22871 (2016)
...Kikhney AG, Müller IB, Eberle RJ, Oberthür D, Cang H, Svergun DI, Perbandt M, Betzel C, Wrenger C
RgGuinier 3.0 nm
Dmax 9.0 nm
VolumePorod 145 nm3

SASDAY8 – ...alphacoronavirus non-structural protein Nsp10 in the presence of Zn

HCoV-229E Non-structural protein 10 experimental SAS data
CORAL model
Sample: ...al protein 10 monomer, 15 kDa Human coronavirus 229E protein
Buffer: 25 mM HEPES 280 mM NaCl 2 mM DTT 500 µM ZnCl2, pH: 7.6
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 25
...alphacoronavirus non-structural protein Nsp10
Sven ...alke, Al Kikhney
RgGuinier 1.7 nm
Dmax 5.8 nm

SASDA39 – HCoV-229E Nsp10 in the absence of Zn

HCoV-229E Non-structural protein 10 experimental SAS data
CORAL model
Sample: ...al protein 10 monomer, 15 kDa Human coronavirus 229E protein
Buffer: 25 mM HEPES 400 mM NaCl 1 mM EDTA 5% glycerol 40 mM NaH2PO4, pH: 7.9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2011 Sep 8
...alphacoronavirus non-structural protein Nsp10
Sven ...alke, Al Kikhney
RgGuinier 1.9 nm
Dmax 6.9 nm

SASDEP3 – Senescence-associated E3 ubiquitin ligase 1, 7.3 mg/ml

Senescence-associated E3 ubiquitin ligase 1 experimental SAS data
SASREF MX model
Sample: Senescence-associated E3 ubiquitin ligase 1 tetramer, 355 kDa ...aliana protein
Buffer: 50 mM Tris, 250 mM NaCl, pH: 9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Senescence-associated ubiquitin ligase 1 (SAUL1)
Haifa El Kilani, Al Kikhney
RgGuinier 6.5 nm
Dmax 24.0 nm

SASDAC6 – ...al binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein experimental SAS data
PsrP functional binding region Rg histogram
Sample: ...al binding region (187-385) of the pneumococcal serine-rich repeat protein monomer, 22 kDa Streptococcus pneumoniae protein
Buffer: PBS 5 % Glycerol, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2013 Feb 27
...al aggregation; structural insights into pneumococcal biofilm formation. Sci Rep 6:32371 (2016)
...Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A
RgGuinier 2.0 nm
Dmax 7.8 nm
VolumePorod 38 nm3

SASDA49 – Cation-free slp-B53

S-layer protein experimental SAS data
DAMMIF model
Sample: S-layer protein monomer, 116 kDa Lysinibacillus sphaericus protein
Buffer: Water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
...alysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus. Eur Biophys J 46(1):77-89 (2017)
...alke S, Drobot B, Oberthuer D, Kikhney A, Guenther T, Fahmy K, Svergun D, Betzel C, Raff J
RgGuinier 5.8 nm
Dmax 22.0 nm
VolumePorod 495 nm3

SASDEQ3 – Senescence-associated E3 ubiquitin ligase 1, 5.6 mg/ml

Senescence-associated E3 ubiquitin ligase 1 experimental SAS data
SASREF MX model
Sample: Senescence-associated E3 ubiquitin ligase 1 tetramer, 355 kDa ...aliana protein
Buffer: 50 mM Tris, 250 mM NaCl, pH: 9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Senescence-associated ubiquitin ligase 1 (SAUL1)
Haifa El Kilani, Al Kikhney
RgGuinier 5.9 nm
Dmax 22.0 nm

SASDAE6 – ...al binding region

Functional binding region (120-395) of the pneumococcal serine-rich repeat protein experimental SAS data
PsrP functional binding region Rg histogram
Sample: ...al binding region (120-395) of the pneumococcal serine-rich repeat protein monomer, 30 kDa Streptococcus pneumoniae protein
Buffer: PBS 5 % Glycerol, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2013 Feb 27
...al aggregation; structural insights into pneumococcal biofilm formation. Sci Rep 6:32371 (2016)
...Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A
RgGuinier 2.9 nm
Dmax 12.5 nm
VolumePorod 41 nm3

SASDA59 – slp-B53 with Ca2+

S-layer protein experimental SAS data
DAMMIF model
Sample: S-layer protein monomer, 116 kDa Lysinibacillus sphaericus protein
Buffer: Water with Ca2+, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
...alysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus. Eur Biophys J 46(1):77-89 (2017)
...alke S, Drobot B, Oberthuer D, Kikhney A, Guenther T, Fahmy K, Svergun D, Betzel C, Raff J
RgGuinier 6.4 nm
Dmax 28.1 nm
VolumePorod 609 nm3

SASDER3 – Senescence-associated E3 ubiquitin ligase 1, 1.8 mg/ml

Senescence-associated E3 ubiquitin ligase 1 experimental SAS data
SASREF MX model
Sample: Senescence-associated E3 ubiquitin ligase 1 tetramer, 355 kDa ...aliana protein
Buffer: 50 mM Tris, 250 mM NaCl, pH: 9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Senescence-associated ubiquitin ligase 1 (SAUL1)
Haifa El Kilani, Al Kikhney
RgGuinier 5.9 nm
Dmax 23.0 nm

SASDA69 – slp-B53 with Mg2+

S-layer protein experimental SAS data
DAMMIF model
Sample: S-layer protein monomer, 116 kDa Lysinibacillus sphaericus protein
Buffer: Water with Mg2+, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
...alysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus. Eur Biophys J 46(1):77-89 (2017)
...alke S, Drobot B, Oberthuer D, Kikhney A, Guenther T, Fahmy K, Svergun D, Betzel C, Raff J
RgGuinier 6.6 nm
Dmax 29.0 nm
VolumePorod 597 nm3

SASDEV3 – Senescence-associated E3 ubiquitin ligase 1, 0.6 mg/ml

Senescence-associated E3 ubiquitin ligase 1 experimental SAS data
SASREF MX model
Sample: Senescence-associated E3 ubiquitin ligase 1 tetramer, 355 kDa ...aliana protein
Buffer: 50 mM Tris, 250 mM NaCl, pH: 9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Senescence-associated ubiquitin ligase 1 (SAUL1)
Haifa El Kilani, Al Kikhney
RgGuinier 4.6 nm
Dmax 17.2 nm
VolumePorod 230 nm3

SASDET3 – Armadillo repeat 7-11 from SAUL1

U-box domain-containing protein 44 experimental SAS data
SASREF MX model
Sample: U-box domain-containing protein 44 tetramer, 185 kDa ...aliana protein
Buffer: 50 mM Tris, 250 mM NaCl, pH: 9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Senescence-associated ubiquitin ligase 1 (SAUL1)
Haifa El Kilani, Al Kikhney
RgGuinier 4.5 nm
Dmax 17.4 nm
VolumePorod 245 nm3

SASDEU3 – GST-SAUL1

Senescence-associated E3 ubiquitin ligase 1Glutathione S-transferase experimental SAS data
CORAL model
Sample: Senescence-associated E3 ubiquitin ligase 1 tetramer, 355 kDa ...aliana protein
Glutathione S-transferase tetramer, 106 kDa Schistosoma japonicum protein
Buffer: 50 mM Tris, 250 mM NaCl, pH: 9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 20
Senescence-associated ubiquitin ligase 1 (SAUL1)
Haifa El Kilani, Al Kikhney
RgGuinier 7.6 nm
Dmax 28.0 nm
VolumePorod 778 nm3