| MWexperimental | 48 | kDa |
| MWexpected | 53 | kDa |
| VPorod | 85 | nm3 |
|
log I(s)
2.19×100
2.19×10-1
2.19×10-2
2.19×10-3
|
s, nm-1
|
Structural and functional studies reveal the molecular basis of substrate promiscuity of a glycosyltransferase originating from a major agricultural pest
Arriaza R, Abiskaroon B, Patel M, Daneshian L, Kluza A, Snoeck S, Watkins M, Hopkins J, Van Leeuwen T, Grbic M, Grbic V, Borowski T, Chruszcz M, Journal of Biological Chemistry :105421 (2023) DOISASDSH5 – UDP-glycosyltransferase 202A2 from Tetranychus urticae
UDP-glycosyltransferase 202A2|
|
|
Fits and models
|
|
|
|
|
Synchrotron SAXS data from solutions of UDP-glycosyltransferase 202A2 in 20 mM sodium phosphate, 150 mM NaCl, pH 7.8 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 100K detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 500.00 μl sample at 5 mg/ml was injected at a 0.60 ml/min flow rate onto a Cytiva Superdex 200 Increase 10/300 column at 22°C. 2225 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
SEC was run at using an AKTA Pure FPLC (GE) system. SAXS data were collected after the eluate passed through a UV monitor and then flown directly through the SAXS flow cell. The SAXS flow cell consists of a 1.0 mm ID quartz capillary with ~20 µm walls. |
|
|||||||||||||||||||||||||||