Small Angle Scattering Biological Data Bank SASBDB


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16 hits found for Bon

SASDJW3 – YraP from Acinetobacter baumannii full-length, minus signal-peptide and acyl anchoring cysteine

BON domain protein experimental SAS data

Sample:	BON domain protein decamer, 229 kDa Acinetobacter baumannii protein
Buffer:	20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Apr 11

BonA from Acinetobacter baumannii Forms a Divisome-Localized Decamer That Supports Outer Envelope Function. mBio :e0148021 (2021)
Grinter R, Morris FC, Dunstan RA, Leung PM, Kropp A, Belousoff M, Gunasinghe SD, Scott NE, Beckham S, Peleg AY, Greening C, Li J, Heinz E, Lithgow T

R_g^Guinier	4.7	nm
D_max	16.4	nm
Volume^Porod	546	nm³

SASDJX3 – YraP from Acinetobacter baumannii full-length, minus signal-peptide and 27 N-terminal amino acids

Sample:	BON domain protein monomer, 20 kDa Acinetobacter baumannii protein
Buffer:	20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Apr 11

R_g^Guinier	3.1	nm
D_max	10.8	nm
Volume^Porod	49	nm³

SASDPC7 – Ordered core SD1 domain of KNR4

Cell wall assembly regulator SMI1 experimental SAS data

Sample:	Cell wall assembly regulator SMI1 monomer, 31 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	100 mM MES, 300 mM NaCl, pH: 6
Experiment:	SAXS data collected at BM29, ESRF on 2016 Mar 18

The conserved yeast protein Knr4 involved in cell wall integrity is a multi-domain intrinsically disordered protein. J Mol Biol :168048 (2023)
...Bon C, Guillien M, Caisso A, Mourey Funding L, Marie François Funding J, Maveyraud L, Zerbib D

R_g^Guinier	2.3	nm
D_max	8.0	nm
Volume^Porod	62	nm³

SASDPD7 – C-terminal truncated KNR4

Sample:	Cell wall assembly regulator SMI1 monomer, 40 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	100 mM MES, 300 mM NaCl, pH: 6
Experiment:	SAXS data collected at BM29, ESRF on 2017 Mar 4

R_g^Guinier	2.8	nm
D_max	11.0	nm
Volume^Porod	79	nm³

SASDPE7 – Full length KNR4

Sample:	Cell wall assembly regulator SMI1 monomer, 57 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	100 mM MES, 300 mM NaCl, pH: 6
Experiment:	SAXS data collected at BM29, ESRF on 2016 Mar 7

R_g^Guinier	4.8	nm
D_max	17.0	nm
Volume^Porod	117	nm³

SASDNK9 – Monomer of the full-length apo-polyketide synthase Pks13 of Mycobacterium tuberculosis

Polyketide synthase Pks13 experimental SAS data

Sample:	Polyketide synthase Pks13 monomer, 188 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Mar 1

Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L

R_g^Guinier	7.3	nm
D_max	24.8	nm
Volume^Porod	480	nm³

SASDNL9 – ...bon chain

Sample:	Polyketide synthase Pks13 dimer, 376 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2011 Nov 23

R_g^Guinier	10.3	nm
D_max	36.0	nm
Volume^Porod	2300	nm³

SASDNM9 – Thioesterase domain of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 33 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	1.9	nm
D_max	5.5	nm
Volume^Porod	56	nm³

SASDNN9 – di-domain sub fragment ACP2TE of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 62 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	3.2	nm
D_max	12.0	nm
Volume^Porod	80	nm³

SASDNP9 – AT52: acyltransferase domain and insertion domain of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 53 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	3.5	nm
D_max	12.0	nm
Volume^Porod	115	nm³

SASDNQ9 – Acyltransferase domain of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 51 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2011 Jun 3

R_g^Guinier	2.8	nm
D_max	8.0	nm
Volume^Porod	85	nm³

SASDNR9 – Subfragment ACP1KSAT tridomain of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 116 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl 50 mM NaCl, pH: 8.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	3.8	nm
D_max	12.9	nm
Volume^Porod	186	nm³

SASDNS9 – KS domain of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 51 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 Dec 7

R_g^Guinier	2.9	nm
D_max	10.0	nm
Volume^Porod	82	nm³

SASDNT9 – Subfragment KSAT of Polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 104 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2008 Sep 20

R_g^Guinier	3.8	nm
D_max	13.0	nm
Volume^Porod	155	nm³

SASDNU9 – Mycocerosic acid synthase in its apo form and at neutral pH

Mycocerosic acid synthase experimental SAS data

Sample:	Mycocerosic acid synthase monomer, 224 kDa Mycobacterium bovis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, 10% glycerol, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Oct 1

R_g^Guinier	5.7	nm
D_max	21.0	nm
Volume^Porod	420	nm³

SASDNV9 – Phenolpthiocerol synthase A of BCG at neutral pH

Phenolpthiocerol synthesis type-I polyketide synthase ppsA experimental SAS data

Sample:	Phenolpthiocerol synthesis type-I polyketide synthase ppsA monomer, 199 kDa Mycobacterium bovis (strain … protein
Buffer:	50 mM Tris-HCl, 500 mM NaCl, 2 mM EDTA, 10% glycerol, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 Jun 14

R_g^Guinier	6.7	nm
D_max	20.2	nm
Volume^Porod	680	nm³

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