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3 hits found for Cyr

SASDHC2 – PieE-FAD: 2,4-dichlorophenol 6-monooxygenase bound to flavin adenine dinucleotide

2,4-dichlorophenol 6-monooxygenaseFlavin adenine dinucleotide experimental SAS data
DAMMIN model
Sample: 2,4-dichlorophenol 6-monooxygenase hexamer, 399 kDa Streptomyces sp. SCSIO … protein
Flavin adenine dinucleotide hexamer, 5 kDa
Buffer: 20 mM Tris, 150 mM NaCl, 5 mM DTT, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, Département de Biochimie, Université de Montréal on 2019 Oct 22
Structural analyses of the group A flavin-dependent monooxygenase PieE reveal a sliding FAD cofactor conformation bridging OUT and IN conformations. J Biol Chem (2020)
...Cyr N, Zhu X, Barma J, Pascal JM, Couture M, Zhang C, Shi R
RgGuinier 4.8 nm
Dmax 13.2 nm
VolumePorod 624 nm3

SASDFR7 – Rabbit muscle fructose-bisphosphate aldolase A, K229M mutant

Fructose-bisphosphate aldolase A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Fructose-bisphosphate aldolase A tetramer, 157 kDa Oryctolagus cuniculus protein
Buffer: 20 mM HEPES, pH: 7
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, Département de Biochimie, Université de Montréal on 2019 Jun 14
Rabbit muscle fructose-1,6-bisphosphate aldolase K229M mutant
Normand Cyr
RgGuinier 3.6 nm
Dmax 11.8 nm
VolumePorod 213 nm3

SASDP79 – Chromatin assembly factor 1 - long alpha-helix domain

Chromatin assembly factor 1 subunit A experimental SAS data
DAMMIN model
Sample: Chromatin assembly factor 1 subunit A monomer, 18 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.1 mM TCEP, pH: 7
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, Département de Biochimie, Université de Montréal on 2021 Oct 28
Unorthodox PCNA Binding by Chromatin Assembly Factor 1 International Journal of Molecular Sciences 23(19):11099 (2022)
...Cyr N, Sverzhinsky A, Melendy T, Pascal J, Laue E, Borden K, Omichinski J, Verreault A
RgGuinier 4.1 nm
Dmax 17.2 nm
VolumePorod 54 nm3