Small Angle Scattering Biological Data Bank SASBDB


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19 hits found for Fuchs

SASDSA5 – dark-state short-linker LOV-activated diguanylate cyclase

Diguanylate cyclase experimental SAS data

Sample:	Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein
Buffer:	10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 May 4

Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
...Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A

R_g^Guinier	3.4	nm
D_max	13.6	nm
Volume^Porod	102	nm³

SASDSB5 – light-state short-linker LOV-activated diguanylate cyclase

Sample:	Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein
Buffer:	10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 May 4

R_g^Guinier	3.4	nm
D_max	15.7	nm
Volume^Porod	104	nm³

SASDSC5 – dark-state long-linker LOV-activated diguanylate cyclase

Sensor domain-containing diguanylate cyclase experimental SAS data

Sample:	Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer:	10 mM Tris, 50 mM NaCl, 2 mM MgCl2, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 May 4

R_g^Guinier	2.9	nm
D_max	11.1	nm
Volume^Porod	115	nm³

SASDSD5 – light-state long-linker LOV-activated diguanylate cyclase

Sensor domain-containing diguanylate cyclase Kratky plot

Sample:	Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer:	10 mM Tris, 50 mM NaCl, 2 mM MgCl2, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 May 4

R_g^Guinier	3.5	nm
D_max	13.8	nm
Volume^Porod	123	nm³

SASDL73 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 2 mg/mL + UFM1 0.5 mg/mL)

Ubiquitin-like modifier-activating enzyme 5Ubiquitin fold modifer 1 experimental SAS data

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism Journal of Structural Biology :107796 (2021)
Fuchs S, Kikhney A, Schubert R, Kaiser C, Liebau E, Svergun D, Betzel C, Perbandt M

R_g^Guinier	2.9	nm
D_max	11.0	nm

SASDL83 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 3 mg/mL + UFM1 0.75 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.0	nm
D_max	12.0	nm

SASDL93 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 4 mg/mL + UFM1 1.0 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.1	nm
D_max	13.0	nm

SASDLA3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 5 mg/mL + UFM1 1.25 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.1	nm
D_max	13.0	nm
Volume^Porod	100	nm³

SASDLB3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 6 mg/mL + UFM1 1.5 mg/mL)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.1	nm
D_max	13.0	nm
Volume^Porod	103	nm³

SASDLC3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 7 mg/mL + UFM1 1.75 mg/mL)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.1	nm
D_max	13.0	nm
Volume^Porod	104	nm³

SASDLD3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 8 mg/mL + UFM1 2 mg/mL)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.1	nm
D_max	13.0	nm
Volume^Porod	106	nm³

SASDLE3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 9 mg/mL + UFM1 2.25 mg/mL)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.2	nm
D_max	14.5	nm
Volume^Porod	111	nm³

SASDGG6 – Ubiquitin activating enzyme 5 (0.3 mg/ml)

Ubiquitin-like modifier-activating enzyme 5 experimental SAS data

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	2.8	nm
D_max	9.0	nm
Volume^Porod	74	nm³

SASDGH6 – Ubiquitin activating enzyme 5 (1.0 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	2.9	nm
D_max	10.0	nm
Volume^Porod	82	nm³

SASDGM6 – Ubiquitin activating enzyme 5 (1.9 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	2.9	nm
D_max	9.8	nm
Volume^Porod	94	nm³

SASDGL6 – Ubiquitin activating enzyme 5 (3.6 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	97	nm³

SASDGK6 – Ubiquitin activating enzyme 5 (4.4 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	3.1	nm
D_max	12.0	nm
Volume^Porod	104	nm³

SASDGJ6 – Ubiquitin activating enzyme 5 (6.8 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	3.2	nm
D_max	12.0	nm
Volume^Porod	108	nm³

SASDM82 – Ubiquitin-fold modifier 1

Ubiquitin fold modifer 1 experimental SAS data

Sample:	Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	1.5	nm
D_max	5.1	nm
Volume^Porod	16	nm³

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