Small Angle Scattering Biological Data Bank SASBDB


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11 hits found for Garcia-Rodriguez

SASDPK3 – Vibrio cholerae ParD2:ParE2 complex bound to 21-bp DNA operator box

Antitoxin ParDToxin21-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 21-bp DNA operator fragment monomer, 13 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
Garcia-Rodriguez G, Girardin Y, Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	140	nm³

SASDPL3 – Vibrio cholerae ParD2:ParE2 complex bound to 31-bp DNA operator box

Antitoxin ParDToxin31-bp DNA operator box experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 31-bp DNA operator box monomer, 19 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	160	nm³

SASDPM3 – Vibrio cholerae ParD2:ParE2 complex bound to 33-bp DNA operator fragment

Antitoxin ParDToxin33-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 33-bp DNA operator fragment monomer, 20 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	150	nm³

SASDPN3 – Vibrio cholerae ParD2:ParE2 antitoxin:toxin complex

Antitoxin ParDToxin experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Mar 6

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	140	nm³

SASDKY6 – ParD2 antitoxin from Vibrio cholerae in low salt pH 8

Sample:	Antitoxin ParD dodecamer, 108 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Entropic pressure controls the oligomerization of the Vibrio cholerae ParD2 antitoxin Acta Crystallographica Section D Structural Biology 77(7):904-920 (2021)
Garcia-Rodriguez G, Girardin Y, Volkov A, Singh R, Muruganandam G, Van Dyck J, Sobott F, Versées W, Charlier D, Loris R

R_g^Guinier	3.4	nm
D_max	13.9	nm
Volume^Porod	190	nm³

SASDHW7 – RnlA-RnlB toxin-antitoxin complex (mRNA endoribonuclease toxin LS : Antitoxin RnlB)

mRNA endoribonuclease toxin LSAntitoxin RnlB (C-terminal His-tagged) experimental SAS data

mRNA endoribonuclease toxin LS Antitoxin RnlB (C-terminal His-tagged) Kratky plot

Sample:	mRNA endoribonuclease toxin LS dimer, 80 kDa Escherichia coli protein Antitoxin RnlB (C-terminal His-tagged) monomer, 15 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R

R_g^Guinier	3.5	nm
D_max	10.0	nm
Volume^Porod	161	nm³

SASDHX7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS)

mRNA endoribonuclease toxin LS experimental SAS data

mRNA endoribonuclease toxin LS Kratky plot

Sample:	mRNA endoribonuclease toxin LS dimer, 80 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.7	nm
D_max	10.7	nm
Volume^Porod	134	nm³

SASDKL2 – NRD-HEPN - mRNA endoribonuclease toxin LS from Escherichia coli (strain K12)

NRD-HEPN truncated variant of RnlA endoribonuclease experimental SAS data

Sample:	NRD-HEPN truncated variant of RnlA endoribonuclease dimer, 62 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jun 27

R_g^Guinier	3.0	nm
D_max	10.4	nm
Volume^Porod	88	nm³

SASDHY7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS) - R255A single alanine mutant

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged experimental SAS data

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged Kratky plot

Sample:	mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.7	nm
D_max	20.1	nm
Volume^Porod	133	nm³

SASDKS9 – mRNA endoribonuclease toxin LS (D245R mutant); batch-SAXS measurements in low salt buffer

mRNA endoribonuclease toxin LS (D245R mutant) experimental SAS data

Sample:	mRNA endoribonuclease toxin LS (D245R mutant) dimer, 84 kDa Escherichia coli (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

R_g^Guinier	4.3	nm
D_max	15.9	nm
Volume^Porod	183	nm³

SASDHZ7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS) - R318A single alanine mutant

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged experimental SAS data

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged Kratky plot

Sample:	mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.4	nm
D_max	12.7	nm
Volume^Porod	124	nm³

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