| MWexperimental | 78 | kDa |
| MWexpected | 78 | kDa |
| VPorod | 122 | nm3 |
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log I(s)
3.09×100
3.09×10-1
3.09×10-2
3.09×10-3
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s, nm-1
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Structural Characterization of Full-Length Human Dehydrodolichyl Diphosphate Synthase Using an Integrative Computational and Experimental Approach.
Lisnyansky Bar-El M, Lee SY, Ki AY, Kapelushnik N, Loewenstein A, Chung KY, Schneidman-Duhovny D, Giladi M, Newman H, Haitin Y, Biomolecules 9(11) (2019) Europe PMCSASDHX2 – Dehydrodolichyl diphosphate synthase complex subunit DHDDS
Dehydrodolichyl diphosphate synthase complex subunit DHDDS|
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Fits and models
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Synchrotron SAXS data from solutions of DHDDS in Tris-HCl, 150 mM NaCl, 20 mM 2-mercaptoethanol and 0.02% Triton-X100, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 8 mg/ml was injected onto a GE Superdex 200 Increase 10/300 column at 20°C. 2000 successive 1 second frames were collected through the SEC-elution profile. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from the scattering data measured from the sample elution peak.
Flow rate = UNKNOWN |
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