Small Angle Scattering Biological Data Bank SASBDB


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6 hits found for Hutin

SASDG44 – Adenovirus Ad3 fibre knob in complex with the EC2-EC3 fragment of desmoglein 2

Human adenovirus serotype 3 fibre knobHuman desmoglein 2 extracellular domain 2 and 3 experimental SAS data

Sample:	Human adenovirus serotype 3 fibre knob trimer, 69 kDa Human adenovirus serotype … protein Human desmoglein 2 extracellular domain 2 and 3 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 3 mM CaCl2, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Jan 26

Intermediate-resolution crystal structure of the human adenovirus B serotype 3 fibre knob in complex with the EC2-EC3 fragment of desmoglein 2. Acta Crystallogr F Struct Biol Commun 75(Pt 12):750-757 (2019)
...Hutin S, Fender P, Burmeister WP

R_g^Guinier	3.4	nm
D_max	11.2	nm
Volume^Porod	188	nm³

SASDBU4 – Vaccinia primase D5 protein fragment containing the D5N and helicase domain

Primase D5 protein fragment containing the D5N and helicase domain experimental SAS data

Sample:	Primase D5 protein fragment containing the D5N and helicase domain hexamer, 321 kDa Vaccinia virus protein
Buffer:	20 mM Tris 150 mM NaCL 5% glycerol 1mM DTT, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2014 Sep 26

Domain Organization of Vaccinia Virus Helicase-Primase D5. J Virol 90(9):4604-4613 (2016)
Hutin S, Ling WL, Round A, Effantin G, Reich S, Iseni F, Tarbouriech N, Schoehn G, Burmeister WP

R_g^Guinier	4.8	nm
D_max	14.5	nm
Volume^Porod	570	nm³

SASDCM5 – Vaccinia virus binary E9 polymerase / DNA complex

DNA polymerase E9 exo- mutantDNA oligomer template-primer hairpin experimental SAS data

Sample:	DNA polymerase E9 exo- mutant monomer, 117 kDa Vaccinia virus protein DNA oligomer template-primer hairpin monomer, 9 kDa synthetic construct DNA
Buffer:	20 mM Tris HCl, 100 mM NaCl, 4 mM DTT, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2015 Mar 12

The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding. Nat Commun 8(1):1455 (2017)
...Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F

R_g^Guinier	3.5	nm
D_max	12.0	nm
Volume^Porod	210	nm³

SASDCN5 – Vaccinia virus DNA polymerase

Sample:	DNA polymerase E9 monomer, 117 kDa Vaccinia virus protein
Buffer:	20 mM Tris HCl, 100 mM NaCl, 4 mM DTT, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2014 Nov 27

R_g^Guinier	3.9	nm
D_max	12.0	nm
Volume^Porod	202	nm³

SASDCP5 – Vaccinia virus DNA polymerase processivity factor component A20 (C-terminal fragment)

DNA polymerase processivity factor component A20 C-ter fragment experimental SAS data

Sample:	DNA polymerase processivity factor component A20 C-ter fragment monomer, 17 kDa Vaccinia virus protein
Buffer:	25 mM Tris-HCl, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Feb 5

R_g^Guinier	2.2	nm
D_max	7.4	nm
Volume^Porod	31	nm³

SASDSW8 – Apo form of vaccinia virus polymerase holoenzyme E9-A20-D4

DNA polymeraseDNA polymerase processivity factor component OPG148Uracil-DNA glycosylase experimental SAS data

DNA polymerase DNA polymerase processivity factor component OPG148 Uracil-DNA glycosylase Kratky plot

Sample:	DNA polymerase monomer, 117 kDa Vaccinia virus (strain … protein DNA polymerase processivity factor component OPG148 monomer, 49 kDa Vaccinia virus (strain … protein Uracil-DNA glycosylase monomer, 27 kDa Vaccinia virus (strain … protein
Buffer:	200 mM NaCl, 20 mM Tris-HCl and 1 mM TCEP, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jan 25

Structure and flexibility of the DNA polymerase holoenzyme of vaccinia virus. PLoS Pathog 20(5):e1011652 (2024)
...Hutin S, Kraft C, Grimm C, Böttcher B, Fischer U, Tarbouriech N, Iseni F