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Synchrotron SAXS data from solutions of Botulinum Toxin E in 10 mM sodium acetate (20 mM ionic strength), pH 4 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.11 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 4 mg/ml was injected at a 0.10 ml/min flow rate onto a Shodex KW403 column at 20°C. 640 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Botulinum Toxin E at acidic pH (10 mM sodium acetate, 20 mM ionic strength, pH 4). Chi-squared value is an R-Factor as calculated by SASSIE and is given as a percentage (i.e. 5.6%). A perfect fit would give and R-Factor of 0%.
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s, nm-1
