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6 hits found for Michael Lerche

SASDNX6 – ASO1 DNA in the absence of target RNA

LNA-DNA-LNA experimental SAS data
LNA-DNA-LNA Kratky plot
Sample: LNA-DNA-LNA monomer, 4 kDa DNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.3 nm
Dmax 4.0 nm
VolumePorod 4 nm3

SASDNY6 – ASO2 DNA in the absence of target RNA

MOE PS gapmers 3-8-3 experimental SAS data
DAMMIF model
Sample: MOE PS gapmers 3-8-3 monomer, 4 kDa DNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.4 nm
Dmax 4.3 nm
VolumePorod 5 nm3

SASDNZ6 – ASO3 RNA in the absence of target RNA

oME ASO experimental SAS data
oME ASO Kratky plot
Sample: oME ASO monomer, 4 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.4 nm
Dmax 4.0 nm
VolumePorod 7 nm3

SASDN27 – ASO1 DNA in the presence target RNA

LNA-DNA-LNAPSCK9, 24mer ASO binding site experimental SAS data
LNA-DNA-LNA PSCK9, 24mer ASO binding site Kratky plot
Sample: LNA-DNA-LNA monomer, 4 kDa DNA
PSCK9, 24mer ASO binding site monomer, 8 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 15 nm3

SASDN37 – ASO2 DNA in the presence of target RNA

MOE PS gapmers 3-8-3PSCK9, 24mer ASO binding site experimental SAS data
DAMMIF model
Sample: MOE PS gapmers 3-8-3 monomer, 4 kDa DNA
PSCK9, 24mer ASO binding site monomer, 8 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 2.1 nm
Dmax 6.4 nm
VolumePorod 16 nm3

SASDN47 – ASO3 RNA in the presence of target RNA

oME ASOPSCK9, 24mer ASO binding site experimental SAS data
oME ASO PSCK9, 24mer ASO binding site Kratky plot
Sample: oME ASO monomer, 4 kDa RNA
PSCK9, 24mer ASO binding site monomer, 8 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 16 nm3