| MWexperimental | 30 | kDa |
| MWexpected | 33 | kDa |
| VPorod | 38 | nm3 |
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log I(s)
1.97×10-2
1.97×10-3
1.97×10-4
1.97×10-5
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s, nm-1
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Structure of a putative immature form of a Rieske-type iron-sulfur protein in complex with zinc chloride.
Tsutsumi E, Niwa S, Takeda R, Sakamoto N, Okatsu K, Fukai S, Ago H, Nagao S, Sekiguchi H, Takeda K, Commun Chem 6(1):190 (2023) Europe PMCSASDSV5 – Dimeric structure of Zn-TtPetA (Ubiquinol-cytochrome c reductase)
Ubiquinol-cytochrome c reductase iron-sulfur subunit|
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Fits and models
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Synchrotron SAXS data from solutions of Zn-TtPetA in 10 mM Tris-HCl pH 7.6, 150 mM NaCl, 5% glycerol, were collected on the BL38B1 beam line at SPring-8 (Hyogo prefecture, Japan) using a PILATUS 3 S 2M detector at a sample-detector distance of 2.6 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 200.00 μl sample at 7 mg/ml was injected at a 0.30 ml/min flow rate onto a Cytiva Superdex 200 Increase 10/300 column at 20°C. 32 successive 3 second frames were collected through the SEC sample elution peak and used for averaging. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN |
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