SAXS data from solutions of the CALX1.2 CBD12 domain in the Ca2+-free (Apo) state in 20 mM TRIS, 200 mM NaCl, 1% v/v glycerol, 0.03% w/v NaN3, 1 mM β-mercaptoethanol, 2 mM EDTA, pH 7.5 were collected using a Xenocs Xeuss instrument at the University of São Paulo Institute of Physics (São Paulo, Brazil) equipped with a Pilatus 300K detector at a sample-detector distance of 0.8 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 3 and 3 mg/ml were measured at 35°C. Four successive 1800 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
s, nm-1
