Structural analyses of PCNA from the fungal pathogen Candida albicans identify three regions with species-specific conformations.

Sundaram R, Manohar K, Patel SK, Acharya N, Vasudevan D, FEBS Lett (2021) Europe PMC

SASDHQ9 – Proliferating cell nuclear antigen (PCNA) from pathogenic yeast Candida albicans

Proliferating cell nuclear antigen (C-terminal His-tagged)
MWI(0) 92 kDa
MWexpected 90 kDa
VPorod 128 nm3
log I(s) 8.72×101 8.72×100 8.72×10-1 8.72×10-2
Proliferating cell nuclear antigen (C-terminal His-tagged) small angle scattering data  s, nm-1
ln I(s)
Proliferating cell nuclear antigen (C-terminal His-tagged) Guinier plot ln 8.72×101 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Proliferating cell nuclear antigen (C-terminal His-tagged) Kratky plot 1.104 0 √3 sRg
p(r)
Proliferating cell nuclear antigen (C-terminal His-tagged) pair distance distribution function Rg: 3.5 nm 0 Dmax: 10.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Proliferating cell nuclear antigen (C-terminal His-tagged) GASBOR model
Synchrotron SAXS data from solutions of proliferating cell nuclear antigen (PCNA) in 20 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM DTT were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0998 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Proliferating cell nuclear antigen (C-terminal His-tagged) (PCNA or POL30)
Mol. type   Protein
Organism   Candida albicans
Olig. state   Trimer
Mon. MW   30.1 kDa
 
UniProt   Q5AMN0 (1-259)
Sequence   FASTA