Disordered regions in proteusin peptides guide post-translational modification by a flavin-dependent RiPP brominase.

Nguyen NA, Vidya FNU, Yennawar NH, Wu H, McShan AC, Agarwal V, Nat Commun 15(1):1265 (2024) Europe PMC

SASDTM5 – Proteusin peptide

NHLP leader peptide family natural product (I56V)
MWexperimental 12 kDa
MWexpected 9 kDa
VPorod 39 nm3
log I(s) 7.49×100 7.49×10-1 7.49×10-2 7.49×10-3
NHLP leader peptide family natural product (I56V) small angle scattering data  s, nm-1
ln I(s)
NHLP leader peptide family natural product (I56V) Guinier plot ln 7.49×100 Rg: 1.9 nm 0 (1.9 nm)-2 s2
(sRg)2I(s)/I(0)
NHLP leader peptide family natural product (I56V) Kratky plot 1.104 0 3 sRg
Dmax: 5.5 nm

Data validation


There are no models related to this curve.

SAXS data from solutions of proteusin peptide in 20 mM sodium phosphate, pH 7.5, 100 mM NaCl and 48 µM FAD were collected using a Rigaku BioSAXS2000 2D-Kratky instrument (The Pennsylvania State University, University Park PA, USA) equipped with a HyPix-3000 Hybrid Photon Counting (HPC) detector detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 5°C. One 3600 second frame was collected. The data were normalized to the intensity of the transmitted beam and the scattering of the solvent-blank was subtracted.

AlphaFold Protein Structure Database entry (monomer): https://alphafold.ebi.ac.uk/entry/A0A1Z4BZH1.

NHLP leader peptide family natural product (I56V) (Proteusin peptide)
Mol. type   Protein
Organism   Methylovulum psychrotolerans
Olig. state   Monomer
Mon. MW   9.3 kDa
 
UniProt   A0A1Z4BZH1 (1-81)
Sequence   FASTA