Full length of DnaG primase from Bacillus subtilis

Zhongchuan Liu.

SASDEA8 – Full length of DnaG (DNA Primase) from Bacillus subtilis

DNA primase
MWI(0) 70 kDa
MWexpected 69 kDa
VPorod 119 nm3
log I(s) 8.92×101 8.92×100 8.92×10-1 8.92×10-2
DNA primase small angle scattering data  s, nm-1
ln I(s)
DNA primase Guinier plot ln 8.92×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
DNA primase Kratky plot 1.104 0 √3 sRg
p(r)
DNA primase pair distance distribution function Rg: 3.9 nm 0 Dmax: 14.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
DNA primase CORAL model
log I(s)
 s, nm-1
DNA primase DAMMIF model
Synchrotron SAXS data from solutions of full length of DnaG (DNA Primase) from Bacillus subtilis in 25 mM Tris-HCl,100 mM NaCl, pH 8 were collected on the NCPSS BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Dectris Pilatus 1M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.154 nm in the momentum transfer range of 0.2 < s < 4 1/nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 7 mg/ml were measured at 10°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

DNA primase
Mol. type   Protein
Organism   Bacillus subtilis
Olig. state   Monomer
Mon. MW   68.7 kDa
 
UniProt   P05096
Sequence   FASTA