Small Angle Scattering Biological Data Bank SASBDB


Advanced search

82 hits found for Zielinski

SASDRE3 – Tissue Transglutaminase + Ca: Time-resolved 0ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data

Protein-glutamine gamma-glutamyltransferase 2 Kratky plot

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L

R_g^Guinier	4.1	nm
D_max	16.0	nm
Volume^Porod	150	nm³

SASDRF3 – Tissue Transglutaminase + Ca: Time-resolved 32 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.1	nm
D_max	17.0	nm
Volume^Porod	170	nm³

SASDRG3 – Tissue Transglutaminase + Ca: Time-resolved 63 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.1	nm
D_max	18.0	nm
Volume^Porod	155	nm³

SASDRH3 – Tissue Transglutaminase + Ca: Time-resolved 100 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.1	nm
D_max	20.0	nm
Volume^Porod	190	nm³

SASDRJ3 – Tissue Transglutaminase + Ca: Time-resolved 316 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.5	nm
D_max	24.0	nm
Volume^Porod	200	nm³

SASDRK3 – Tissue Transglutaminase + Ca: Time-resolved 631 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.6	nm
D_max	26.0	nm
Volume^Porod	215	nm³

SASDRL3 – Tissue Transglutaminase + Ca: Time-resolved 1500 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.6	nm
D_max	28.0	nm
Volume^Porod	230	nm³

SASDTL3 – Tissue Transglutaminase: Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.2	nm
D_max	20.0	nm
Volume^Porod	148	nm³

SASDRM3 – Tissue Transglutaminase + Ca: 5min Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29

R_g^Guinier	5.0	nm
D_max	27.0	nm
Volume^Porod	241	nm³

SASDTM3 – Tissue Transglutaminase + 25 µM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.8	nm
D_max	17.5	nm
Volume^Porod	132	nm³

SASDRN3 – Tissue Transglutaminase + Ca: 10min Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	5.5	nm
D_max	26.0	nm
Volume^Porod	300	nm³

SASDTN3 – Tissue Transglutaminase + 50 µM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.7	nm
D_max	17.5	nm
Volume^Porod	124	nm³

SASDRP3 – Tissue Transglutaminase + Ca: 30min Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29

R_g^Guinier	6.9	nm
D_max	31.5	nm
Volume^Porod	490	nm³

SASDTP3 – Tissue Transglutaminase + 100 µM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.6	nm
D_max	18.0	nm
Volume^Porod	121	nm³

SASDRQ3 – Trypsin + Aprotinin: Time-Resolved 10 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data

Serine protease 1 Pancreatic trypsin inhibitor Kratky plot

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	23	nm³

SASDTQ3 – Tissue Transglutaminase + 250 µM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.5	nm
D_max	17.5	nm
Volume^Porod	120	nm³

SASDRR3 – Trypsin + Aprotinin: Time-Resolved 30 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	28	nm³

SASDTR3 – Tissue Transglutaminase + 500 µM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.4	nm
D_max	18.0	nm
Volume^Porod	118	nm³

SASDRS3 – Trypsin + Aprotinin: Time-Resolved 100 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	1.8	nm
D_max	5.3	nm
Volume^Porod	30	nm³

SASDTS3 – Tissue Transglutaminase + 1 mM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.4	nm
D_max	17.0	nm
Volume^Porod	115	nm³

SASDRT3 – Trypsin + Aprotinin: Time-Resolved 400 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	27	nm³

SASDTT3 – Tissue Transglutaminase + 2 mM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.3	nm
D_max	17.0	nm
Volume^Porod	111	nm³

SASDRU3 – Trypsin + Aprotinin: Time-Resolved 630 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	25	nm³

SASDTU3 – Tissue Transglutaminase + 5 mM GTP: Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 5 mM GTP, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.2	nm
D_max	14.0	nm
Volume^Porod	108	nm³

SASDRV3 – Trypsin + Aprotinin: Time-Resolved 1000 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	1.8	nm
D_max	5.3	nm
Volume^Porod	28	nm³

SASDTV3 – Tissue Transglutaminase + 5 mM GDP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.5	nm
D_max	17.5	nm
Volume^Porod	120	nm³

SASDRW3 – Trypsin + Aprotinin: Time-Resolved 2000 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.0	nm
D_max	5.3	nm
Volume^Porod	29	nm³

SASDTW3 – Tissue Transglutaminase + 250 µM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.3	nm
D_max	21.0	nm
Volume^Porod	160	nm³

SASDRX3 – Trypsin + Aprotinin: Complex equilibrium

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	31	nm³

SASDTX3 – Tissue Transglutaminase + 500 µM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.3	nm
D_max	20.0	nm
Volume^Porod	158	nm³

SASDRY3 – Kenics GAC rRNA + Mg: Time-Resolved 10 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.2	nm
D_max	10.0	nm
Volume^Porod	28	nm³

SASDTY3 – Tissue Transglutaminase + 1 mM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.5	nm
D_max	24.0	nm
Volume^Porod	180	nm³

SASDRZ3 – Kenics GAC rRNA + Mg: Time-Resolved 32 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.2	nm
D_max	10.0	nm
Volume^Porod	27	nm³

SASDTZ3 – Tissue Transglutaminase + 2 mM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	5.3	nm
D_max	25.5	nm
Volume^Porod	234	nm³

SASDR24 – Kenics GAC rRNA + Mg: Time-Resolved 63 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.2	nm
D_max	9.5	nm
Volume^Porod	27	nm³

SASDT24 – Tissue Transglutaminase + 50 µM inhibitor LM11

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.3	nm
D_max	20.0	nm
Volume^Porod	158	nm³

SASDR34 – Kenics GAC rRNA + Mg: Time-Resolved 100 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.2	nm
D_max	10.0	nm
Volume^Porod	28	nm³

SASDT34 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 250 µM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.2	nm
D_max	19.0	nm
Volume^Porod	155	nm³

SASDR44 – Kenics GAC rRNA + Mg: Time-Resolved 316 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.2	nm
D_max	12.0	nm
Volume^Porod	27	nm³

SASDT44 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 500 µM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.9	nm
D_max	25.0	nm
Volume^Porod	191	nm³

SASDR54 – Kenics GAC rRNA + Mg: Time-Resolved 631 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.3	nm
D_max	9.8	nm
Volume^Porod	26	nm³

SASDT54 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 1 mM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.7	nm
D_max	28.0	nm
Volume^Porod	198	nm³

SASDR64 – Kenics GAC rRNA + Mg: Time-Resolved 1000 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.3	nm
D_max	11.0	nm
Volume^Porod	26	nm³

SASDT64 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 2 mM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	5.2	nm
D_max	25.0	nm
Volume^Porod	236	nm³

SASDR74 – Kenics GAC rRNA + Mg: Equilibrium initial

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.4	nm
D_max	10.0	nm
Volume^Porod	32	nm³

SASDT74 – Tissue Transglutaminase + CaCl2 + inhibitor LM11: Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM LM11, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	6.0	nm
D_max	36.0	nm
Volume^Porod	431	nm³

SASDR84 – Kenics GAC rRNA + Mg: Equilibrium final

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.2	nm
D_max	10.0	nm
Volume^Porod	26	nm³

SASDT84 – Tissue Transglutaminase + inhibitor LM11 + CaCl2: Time-resolved 100 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.6	nm
D_max	32.0	nm
Volume^Porod	242	nm³

SASDR94 – GAC rRNA + L11 Protein: Time-Resolved 30 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.4	nm
D_max	12.0	nm
Volume^Porod	34	nm³

SASDT94 – Tissue Transglutaminase + inhibitor LM11 + CaCl2: Time-resolved 316 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.8	nm
D_max	25.0	nm
Volume^Porod	203	nm³

SASDRA4 – GAC rRNA + L11 Protein: Time-Resolved 50 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.5	nm
D_max	13.5	nm
Volume^Porod	36	nm³

SASDTA4 – Tissue Transglutaminase + inhibitor LM11 + CaCl2: Time-resolved 631 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	5.7	nm
D_max	33.0	nm
Volume^Porod	305	nm³

SASDRB4 – GAC rRNA + L11 Protein: Time-Resolved 63 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.4	nm
D_max	11.5	nm
Volume^Porod	33	nm³

SASDTB4 – Tissue Transglutaminase + CaCl2 + inhibitor LM11 + GTP: Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM LM11, 5 mM GTP, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	6.2	nm
D_max	33.0	nm
Volume^Porod	277	nm³

SASDRC4 – GAC rRNA + L11 Protein: Time-Resolved 100 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.4	nm
D_max	11.5	nm
Volume^Porod	35	nm³

SASDTC4 – Tissue Transglutaminase + 50 µM inhibitor 5826 (1% DMSO)

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.1	nm
D_max	19.0	nm
Volume^Porod	144	nm³

SASDRD4 – GAC rRNA + L11 Protein: Time-Resolved 200 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.5	nm
D_max	12.5	nm
Volume^Porod	37	nm³

SASDTD4 – Tissue Transglutaminase + 50 µM inhibitor 5826 (2% DMSO)

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.9	nm
D_max	18.5	nm
Volume^Porod	134	nm³

SASDRE4 – GAC rRNA + L11 Protein: Time-Resolved 316 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.5	nm
D_max	10.0	nm
Volume^Porod	38	nm³

SASDTE4 – Tissue Transglutaminase + 100 µM inhibitor 5826 (2% DMSO)

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.8	nm
D_max	17.6	nm
Volume^Porod	130	nm³

SASDRF4 – GAC rRNA + L11 Protein: Time-Resolved 631 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.5	nm
D_max	13.0	nm
Volume^Porod	38	nm³

SASDTF4 – Tissue Transglutaminase + CaCl2 + inhibitor 5826: Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM 5826 inhibitor, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	6.7	nm
D_max	39.0	nm
Volume^Porod	450	nm³

SASDRG4 – GAC rRNA + L11 Protein: Time-Resolved 1000 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.5	nm
D_max	12.5	nm
Volume^Porod	40	nm³

SASDTG4 – Tissue Transglutaminase + CaCl2 + inhibitor 5826 + GTP: Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM 5826, 5 mM GTP, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	6.0	nm
D_max	29.0	nm
Volume^Porod	248	nm³

SASDRH4 – GAC rRNA + L11 Protein: Time-Resolved 2000 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.6	nm
D_max	12.8	nm
Volume^Porod	39	nm³

SASDTH4 – Tissue Transglutaminase + 1% DMSO

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.0	nm
D_max	18.5	nm
Volume^Porod	139	nm³

SASDRJ4 – GAC rRNA + L11 Protein: SEC-SAXS final

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Nov 19

R_g^Guinier	2.6	nm
D_max	13.0	nm
Volume^Porod	48	nm³

SASDTJ4 – Tissue Transglutaminase + 2% DMSO

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.9	nm
D_max	18.5	nm
Volume^Porod	133	nm³

SASDRK4 – Diffusive GAC rRNA + Mg: Time-Resolved 10 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.2	nm
D_max	12.0	nm
Volume^Porod	29	nm³

SASDTK4 – Tissue Transglutaminase + 5% DMSO

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	3.9	nm
D_max	18.5	nm
Volume^Porod	133	nm³

SASDRL4 – Diffusive GAC rRNA + Mg: Time-Resolved 30 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.2	nm
D_max	12.0	nm
Volume^Porod	29	nm³

SASDTL4 – Tissue Transglutaminase + 10% DMSO

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	4.1	nm
D_max	21.5	nm
Volume^Porod	148	nm³

SASDRM4 – Diffusive GAC rRNA + Mg: Time-Resolved 100 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.2	nm
D_max	9.0	nm
Volume^Porod	29	nm³

SASDTM4 – Tissue Transglutaminase R580K mutant

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data

Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot

Sample:	Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	6.1	nm
D_max	32.0	nm
Volume^Porod	488	nm³

SASDRN4 – Diffusive GAC rRNA + Mg: Time-Resolved 300 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.2	nm
D_max	12.0	nm
Volume^Porod	29	nm³

SASDTN4 – Tissue Transglutaminase R580K mutant + 100 µM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	5.9	nm
D_max	27.0	nm
Volume^Porod	381	nm³

SASDRP4 – Diffusive GAC rRNA + Mg: Time-Resolved 1000 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.4	nm
D_max	13.0	nm
Volume^Porod	30	nm³

SASDTP4 – Tissue Transglutaminase R580K mutant + 1 mM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	5.7	nm
D_max	30.0	nm
Volume^Porod	283	nm³

SASDRQ4 – Diffusive GAC rRNA + Mg: Equilibrium initial

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.4	nm
D_max	10.0	nm
Volume^Porod	34	nm³

SASDTQ4 – Tissue Transglutaminase R580K mutant + 2 mM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	5.3	nm
D_max	25.0	nm
Volume^Porod	264	nm³

SASDRR4 – Diffusive GAC rRNA + Mg: Equilibrium final

50S ribosomal protein L11 experimental SAS data

Sample:	50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.2	nm
D_max	11.0	nm
Volume^Porod	27	nm³

SASDTR4 – Tissue Transglutaminase R580K mutant + 5 mM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

R_g^Guinier	5.2	nm
D_max	25.0	nm
Volume^Porod	243	nm³

Search