eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation.

Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M, Nucleic Acids Res (2023) Europe PMC

SASDQX6 – multimeric eukaryotic initiation factor 4A1 bound to 20 nt (AG)10-24bp duplex RNA

Eukaryotic initiation factor 4A-I
(AG)10-24bp loading-RNA
(AG)10-24bp duplex-RNA
MWexperimental 120 kDa
MWexpected 161 kDa
VPorod 230 nm3
log I(s) 1.52×10-1 1.52×10-2 1.52×10-3 1.52×10-4
Eukaryotic initiation factor 4A-I (AG)10-24bp loading-RNA (AG)10-24bp duplex-RNA small angle scattering data  s, nm-1
ln I(s)
Eukaryotic initiation factor 4A-I (AG)10-24bp loading-RNA (AG)10-24bp duplex-RNA Guinier plot ln 1.53×10-1 Rg: 4.9 nm 0 (4.9 nm)-2 s2
(sRg)2I(s)/I(0)
Eukaryotic initiation factor 4A-I (AG)10-24bp loading-RNA (AG)10-24bp duplex-RNA Kratky plot 1.104 0 √3 sRg
p(r)
Eukaryotic initiation factor 4A-I (AG)10-24bp loading-RNA (AG)10-24bp duplex-RNA pair distance distribution function Rg: 5.1 nm 0 Dmax: 16.2 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of multimeric eukaryotic initiation factor 4A1 bound to 20 nt (AG)10-24bp duplex RNA in 20 mM Hepes, 100 mM KCl, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 4°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Eukaryotic initiation factor 4A-I
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Trimer
Mon. MW   46.2 kDa
 
UniProt   P60842 (1-406)
Sequence   FASTA
 
(AG)10-24bp loading-RNA
Mol. type   RNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   14.6 kDa
Sequence   FASTA
 
(AG)10-24bp duplex-RNA
Mol. type   RNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   7.5 kDa
Sequence   FASTA