| MWexpected | 62 | kDa |
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log I(s)
4.10×10-2
4.10×10-3
4.10×10-4
4.10×10-5
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s, nm-1
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Circularized and solubility‐enhanced MSP
s facilitate simple and high‐yield production of stable nanodiscs for studies of membrane proteins in solution
Johansen N,
Tidemand F,
Nguyen T,
Rand K,
Pedersen M,
Arleth L,
The FEBS Journal
286(9):1734-1751
(2019)
DOISASDQP6 – Nanodisc with POPC and circularized membrane scaffolding protein (csMSP1E3D1)
circularized Membrane scaffolding protein 1 E3 D11-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)
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Fits and models
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![circularized Membrane scaffolding protein 1 E3 D1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) OTHER [STATIC IMAGE] model](/media//pdb_file/SASDQP6_fit1_model1.png "Static model image")
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Synchrotron SAXS data from solutions of nanodics composed of a lipid bilayer of POPC, solubilized by a rim of amphipathic and circularized membrane scaffolding protein (csMSP1E3D1), in 20 mM Tris-HCl pH 7.5, 100 mM NaCl were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Forty 0.045 second frames were collected, at a sample temperature of at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
As decribed in the paper, the nanodiscs have some degree of polydispersity, which were modelled. As part of the post-processing and before model fitting, the data were rebinned to 110 points. |
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