7 hits found for 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid

SASDUS4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (i) Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Nov 30 Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024) Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S RgGuinier 4.8 nm Dmax 16.0 nm VolumePorod 396 nm3

SASDUT4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (ii) Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 30 Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024) Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S RgGuinier 5.5 nm Dmax 27.0 nm

SASDUU4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (ii-remeasured after storage) Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 31 Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024) Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S RgGuinier 8.8 nm Dmax 37.0 nm

SASDUV4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (iii) Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 30 Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024) Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S RgGuinier 4.5 nm Dmax 17.0 nm VolumePorod 556 nm3

SASDUW4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (iv) Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17 Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024) Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S RgGuinier 15.6 nm Dmax 90.0 nm

SASDU25 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) W403L hub domain with PIPA Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17 Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024) Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S RgGuinier 4.4 nm Dmax 15.0 nm VolumePorod 334 nm3

SASDU65 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain with PIPA Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Nov 30 Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024) Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S RgGuinier 4.7 nm Dmax 20.0 nm VolumePorod 375 nm3