Protein quaternary structures in solution are a mixture of multiple forms

Marciano S, Dey D, Listov D, Fleishman S, Sonn-Segev A, Mertens H, Busch F, Kim Y, Harvey S, Wysocki V, Schreiber G, Chemical Science 13(39):11680-11695 (2022) DOI

SASDLR4 – 3-oxoacyl-[acyl-carrier-protein] reductase, FabG

3-oxoacyl-[acyl-carrier-protein] reductase FabG
MWI(0) 93 kDa
MWexpected 26 kDa
VPorod 212 nm3
log I(s) 6.71×10-2 6.71×10-3 6.71×10-4 6.71×10-5
3-oxoacyl-[acyl-carrier-protein] reductase FabG small angle scattering data  s, nm-1
ln I(s)
3-oxoacyl-[acyl-carrier-protein] reductase FabG Guinier plot ln 6.72×10-2 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
3-oxoacyl-[acyl-carrier-protein] reductase FabG Kratky plot 1.104 0 √3 sRg
p(r)
3-oxoacyl-[acyl-carrier-protein] reductase FabG pair distance distribution function Rg: 3.4 nm 0 Dmax: 10.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
3-oxoacyl-[acyl-carrier-protein] reductase FabG PDB (PROTEIN DATA BANK) model
3-oxoacyl-[acyl-carrier-protein] reductase FabG PDB (PROTEIN DATA BANK) model
3-oxoacyl-[acyl-carrier-protein] reductase FabG PDB (PROTEIN DATA BANK) model
3-oxoacyl-[acyl-carrier-protein] reductase FabG SASREF model
Synchrotron SAXS data from solutions of 3-oxoacyl-[acyl-carrier-protein] reductase, FabG. in 50 mM HEPES, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.90 mg/ml was measured at 20°C. 40 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

3-oxoacyl-[acyl-carrier-protein] reductase FabG (FabG)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Unknown
Mon. MW   25.6 kDa
 
UniProt   P0AEK2 (1-244)
Sequence   FASTA
 
PDB ID   1I01
 
PDB ID   1I01
 
PDB ID   1I01
 
PDB ID   101