Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids

Yen-Lin Chen.

SASDKN5 – 30nt Single-stranded RNA

30nt Single-stranded RNA
MWexperimental 10 kDa
MWexpected 10 kDa
log I(s) 4.93×100 4.93×10-1 4.93×10-2 4.93×10-3
30nt Single-stranded RNA small angle scattering data  s, nm-1
ln I(s)
30nt Single-stranded RNA Guinier plot ln 4.93×100 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
30nt Single-stranded RNA Kratky plot 1.104 0 √3 sRg
p(r)
30nt Single-stranded RNA pair distance distribution function Rg: 2.3 nm 0 Dmax: 8.2 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of 30nt Single-stranded RNA in 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH 7 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 S 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.092 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.89 mg/ml was measured at 10°C. 15 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

30nt Single-stranded RNA (ssRNA)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   9.7 kDa
Sequence   FASTA