| MWexperimental | 183 | kDa |
| MWexpected | 183 | kDa |
| VPorod | 477 | nm3 |
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log I(s)
1.73×10-2
1.73×10-3
1.73×10-4
1.73×10-5
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s, nm-1
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Targeting the N-Terminus Domain of the Coronavirus Nucleocapsid Protein Induces Abnormal Oligomerization via Allosteric Modulation
Hsu J, Chen J, Lin S, Hong J, Chen Y, Jeng U, Luo S, Hou M, Frontiers in Molecular Biosciences 9 (2022) DOISASDNH6 – full-length MERS CoV N-protein complexed with 5-(2-fluoroethoxy)-1H-indole (P4-3 compound)
Nucleoprotein5-(2-fluoroethoxy)-1H-indole
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Fits and models
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Synchrotron SAXS
data from solutions of
full-length MERS CoV N-protein complexed with 5-(2-fluoroethoxy)-1H-indole (P4-3 compound)
in
50 mM Tris-HCl, 150 mM NaCl, pH 8.5
were collected
on the
23A beam line
at the Taiwan Photon Source, NSRRC storage ring
(Hsinchu, Taiwan)
using a Pilatus 1M-F detector
at a sample-detector distance of 2.5 m and
at a wavelength of λ = 0.08 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample
at 2 mg/ml was injected at a 0.02 ml/min flow rate
onto a Agilent Bio SEC-3, 300 Å column
at 14.9°C.
36 successive
30 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Quoted experimental molecular weight (183 kDa) cannot be verified from the data. |
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