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4 hits found for 5-methylcytosine-specific restriction enzyme A

...ASDJW2 – ...ation dependent EcoKMcrA restriction endonuclease

5-methylcytosine-specific restriction enzyme A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 5-methylcytosine-specific restriction enzyme A dimer, 65 kDa ...a coli protein
Buffer: ...A, 0.01% (w/v) sodium azide and 1 mM DTT, pH: 7.5
Experiment: ...AXS data collected at EMBL P12, ...A III on ...ay 24
Activity and structure of EcoKMcrA. ...Acids Res 46(18):9829-9841 (2018)
...apinska H, Kowalska M, Zagorskaite E, Manakova E, Slyvka A, Xu SY, Siksnys V, Sasnauskas G, Bochtler M
RgGuinier 3.7 nm
Dmax 13.0 nm
VolumePorod 95 nm3

...ASDJX2 – ...ation dependent EcoKMcrA restriction endonuclease in complex with cognate hemimethylated 12bp oligoduplex

cognate hemimethylated 12-bp oligoduplex5-methylcytosine-specific restriction enzyme A experimental SAS data
SASREF model
Sample: ...ate hemimethylated 12-bp oligoduplex dimer, 15 kDa ...A
5-methylcytosine-specific restriction enzyme A dimer, 65 kDa ...a coli protein
Buffer: ...A, 0.01% (w/v) sodium azide and 1 mM DTT, pH: 7.5
Experiment: ...AXS data collected at EMBL P12, ...A III on ...ay 24
Activity and structure of EcoKMcrA. ...Acids Res 46(18):9829-9841 (2018)
...apinska H, Kowalska M, Zagorskaite E, Manakova E, Slyvka A, Xu SY, Siksnys V, Sasnauskas G, Bochtler M
RgGuinier 3.9 nm
Dmax 13.5 nm
VolumePorod 69 nm3

...ASDJY2 – ...al domain of modification dependent EcoKMcrA restriction endonuclease

5-methylcytosine-specific restriction enzyme A (N-terminal domain) experimental SAS data
OTHER model
Sample: 5-methylcytosine-specific restriction enzyme A (N-terminal domain) monomer, 21 kDa ...a coli protein
Buffer: ...A, 0.01% (w/v) sodium azide, 1 mM DTT, pH: 7.5
Experiment: ...AXS data collected at EMBL P12, ...A III on ...ay 24
Activity and structure of EcoKMcrA. ...Acids Res 46(18):9829-9841 (2018)
...apinska H, Kowalska M, Zagorskaite E, Manakova E, Slyvka A, Xu SY, Siksnys V, Sasnauskas G, Bochtler M
RgGuinier 2.1 nm
Dmax 7.5 nm
VolumePorod 29 nm3

...ASDJZ2 – ...al domain of modification dependent EcoKMcrA restriction endonuclease in complex with cognate hemimethylated 12bp oligoduplex

5-methylcytosine-specific restriction enzyme A (N-terminal domain)cognate hemimethylated 12-bp oligoduplex experimental SAS data
OTHER model
Sample: 5-methylcytosine-specific restriction enzyme A (N-terminal domain) monomer, 21 kDa ...a coli protein
...ate hemimethylated 12-bp oligoduplex monomer, 7 kDa ...A
Buffer: ...A, 0.01% (w/v) sodium azide and 1 mM DTT, pH: 7.5
Experiment: ...AXS data collected at EMBL P12, ...A III on ...ay 24
Activity and structure of EcoKMcrA. ...Acids Res 46(18):9829-9841 (2018)
...apinska H, Kowalska M, Zagorskaite E, Manakova E, Slyvka A, Xu SY, Siksnys V, Sasnauskas G, Bochtler M
RgGuinier 2.1 nm
Dmax 7.5 nm
VolumePorod 29 nm3