Structural basis for adhesion G protein-coupled receptor Gpr126 function

Leon K, Cunningham R, Riback J, Feldman E, Li J, Sosnick T, Zhao M, Monk K, Araç D, Nature Communications 11(1) (2020) DOI

SASDFV9 – Adhesion G-protein coupled receptor G6 - zfGpr126 S2 (-ss) D134A/F135A ECR

Adhesion G-protein coupled receptor G6 S2 D134A/F135A
MWexperimental 95 kDa
MWexpected 86 kDa
VPorod 181 nm3
log I(s) 1.53×102 1.53×101 1.53×100 1.53×10-1
Adhesion G-protein coupled receptor G6 S2 D134A/F135A small angle scattering data  s, nm-1
ln I(s)
Adhesion G-protein coupled receptor G6 S2 D134A/F135A Guinier plot ln 1.54×102 Rg: 4.3 nm 0 (4.3 nm)-2 s2
(sRg)2I(s)/I(0)
Adhesion G-protein coupled receptor G6 S2 D134A/F135A Kratky plot 1.104 0 √3 sRg
p(r)
Adhesion G-protein coupled receptor G6 S2 D134A/F135A pair distance distribution function Rg: 4.3 nm 0 Dmax: 14.8 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Adhesion G-protein coupled receptor G6 - zfGpr126 S2 (-ss) D134A/F135A ECR in 150 mM NaCl, 20 mM HEPES, pH 7.5 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 200.00 μl sample at 2.5 mg/ml was injected at a 0.70 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 22°C. 1185 successive 0.800 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Adhesion G-protein coupled receptor G6 S2 D134A/F135A (Gpr126 D134A/F135A)
Mol. type   Protein
Organism   Danio rerio
Olig. state   Monomer
Mon. MW   86.3 kDa
 
UniProt   C6KFA3 (39-839)
Sequence   FASTA