MWexperimental | 11 | kDa |
MWexpected | 14 | kDa |
VPorod | 18 | nm3 |
log I(s)
2.49×102
2.49×101
2.49×100
2.49×10-1
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s, nm-1
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Synchrotron SAXS
data from solutions of
MucBP domain of the adhesion protein PEPE_0118 from Pediococcus pentosaceus. Northeast Structural Genomics Consortium target id PtR41A
in
5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH 7.5
were collected
on the
BL4-2 beam line
at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring
(Menlo Park, CA, USA)
using a Rayonix MX225-HE detector
at a sample-detector distance of 1.5 m and
at a wavelength of λ = 0.13 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2.5 and 7.7 mg/ml were measured
at 20°C.
20 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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