| MWexperimental | 56 | kDa |
| MWexpected | 81 | kDa |
| VPorod | 71 | nm3 |
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log I(s)
1.32×101
1.32×100
1.32×10-1
1.32×10-2
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s, nm-1
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Comparison of angiopoietin-like protein 3 and 4 reveals structural and mechanistic similarities.
Gunn KH, Gutgsell AR, Xu Y, Johnson CV, Liu J, Neher SB, J Biol Chem :100312 (2021) Europe PMCSASDJL8 – N-terminal Angiopoietin-like protein 3 Trimer
Angiopoietin-related protein 3|
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Fits and models
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Synchrotron SAXS data from solutions of angiopoietin-like protein 3 in 20 mM Tris-HCl pH 7.5, 400 mM NaCl, 2% glycerol, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS, Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.07 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 80.00 μl sample at 6.2 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex KW-800 series column at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Number of frames = UNKNOWN |
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