Extrinsic stabilization of antiviral ACE2-Fc fusion proteins targeting SARS-CoV-2.

Svilenov HL, Delhommel F, Siebenmorgen T, Rührnößl F, Popowicz GM, Reiter A, Sattler M, Brockmeyer C, Buchner J, Commun Biol 6(1):386 (2023) Europe PMC

SASDRU7 – Angiotensin-converting enzyme 2/immunoglobulin-Fc chimera protein (Ace2-Fc) at 3.81 mg/ml

Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4
MWexperimental 259 kDa
MWexpected 217 kDa
VPorod 408 nm3
log I(s) 1.22×101 1.22×100 1.22×10-1 1.22×10-2
Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4 small angle scattering data  s, nm-1
ln I(s)
Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4 Guinier plot ln 1.22×101 Rg: 5.5 nm 0 (5.5 nm)-2 s2
(sRg)2I(s)/I(0)
Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4 Kratky plot 1.104 0 √3 sRg
p(r)
Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4 pair distance distribution function Rg: 5.7 nm 0 Dmax: 18.6 nm

Data validation

There are no models related to this curve.

SAXS data from solutions of Ace2-Fc chimera protein in 50 mM Tris, 150 mM NaCl, pH 7.5 were collected using a Rigaku BioSAXS-1000 instrument at the Technische Universität München (TUM, Garching, Germany) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.155 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.81 mg/ml was measured at 25°C. Eight successive 900 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4 (ACE2-IgG4-Fc)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   108.5 kDa
Sequence   FASTA