LIKE EARLY STARVATION 1 and EARLY STARVATION 1 promote and stabilize amylopectin phase transition in starch biosynthesis.

Liu C, Pfister B, Osman R, Ritter M, Heutinck A, Sharma M, Eicke S, Fischer-Stettler M, Seung D, Bompard C, Abt MR, Zeeman SC, Sci Adv 9(21):eadg7448 (2023) Europe PMC

SASDRR5 – Like Early Starvation 1

At3g55760
MWI(0) 88 kDa
MWexpected 60 kDa
VPorod 121 nm3
log I(s) 2.33×101 2.33×100 2.33×10-1 2.33×10-2
At3g55760 small angle scattering data  s, nm-1
ln I(s)
At3g55760 Guinier plot ln 2.33×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
At3g55760 Kratky plot 1.104 0 √3 sRg
p(r)
At3g55760 pair distance distribution function Rg: 4.0 nm 0 Dmax: 16 nm

Data validation

Fits and models

log I(s)
 s, nm-1
At3g55760 GASBOR model
Synchrotron SAXS data from solutions of like early starvation 1 in 50 mM Tris, 150 mM NaCl, 10% glycerol, 2 mM DTT, pH 8 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a AVIEX PCCD170170 detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 21 mg/ml was injected at a 0.20 ml/min flow rate onto a Agilent Bio SEC-3, 150 Å column at 15°C. 254 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

At3g55760 (LESV1)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Monomer
Mon. MW   59.6 kDa
 
UniProt   Q5EAH9 (57-578)
Sequence   FASTA