| MWexperimental | 49 | kDa |
| MWexpected | 46 | kDa |
| VPorod | 89 | nm3 |
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log I(s)
3.24×10-2
3.24×10-3
3.24×10-4
3.24×10-5
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s, nm-1
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Capturing the Conformational Ensemble of the Mixed Folded Polyglutamine Protein Ataxin-3.
Sicorello A, Różycki B, Konarev PV, Svergun DI, Pastore A, Structure (2020) Europe PMCSASDJ57 – Polyglutamine protein ataxin-3 (Q54)
Ataxin-3 (polyglutamine protein ataxin-3 (Q54))|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of polyglutamine protein ataxin-3 (Q54) in 20 mM sodium phosphate buffer, 2 mM TCEP, 5% glycerol, pH 6.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.4 and 11.4 mg/ml were measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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