Small Angle Scattering Biological Data Bank SASBDB

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9 hits found for Bifunctional protein PutA

SASDCP3 – Proline utilization A from Bdellovibrio bacteriovorus

Bifunctional protein PutA experimental SAS data

Sample:	Bifunctional protein PutA dimer, 219 kDa Bdellovibrio bacteriovorus protein
Buffer:	50 mM Tris, 125 mM NaCl, 1 mM EDTA, and 1 mM tris(3-hydroxypropyl)phosphine (THP) at pH 7.5,, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8

...PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ

R_g^Guinier	4.5	nm
D_max	14.0	nm
Volume^Porod	287	nm³

SASDDP3 – N-propargyl glycine-Inactivated Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) collected by SEC-SAXS

Sample:	Bifunctional protein PutA dimer, 215 kDa Bradyrhizobium diazoefficiens protein
Buffer:	50 mM Tris, 50 mM NaCl, 0.5 mM TCEP, 5% (v/v) glycerol, pH: 7.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jul 16

Redox Modulation of Oligomeric State in Proline Utilization A. Biophys J 114(12):2833-2843 (2018)
Korasick DA, Campbell AC, Christgen SL, Chakravarthy S, White TA, Becker DF, Tanner JJ

R_g^Guinier	4.6	nm
D_max	14.4	nm
Volume^Porod	324	nm³

SASDCQ3 – Proline utilization A from Desulfovibrio vulgaris 1.5 mg/mL

Sample:	Bifunctional protein PutA dimer, 229 kDa Desulfovibrio vulgaris protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8

...PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ

R_g^Guinier	4.4	nm
D_max	16.0	nm
Volume^Porod	293	nm³

SASDDQ3 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) collected by SEC-SAXS

Sample:	Bifunctional protein PutA tetramer, 430 kDa Bradyrhizobium diazoefficiens protein
Buffer:	50 mM Tris, 50 mM NaCl, 0.5 mM TCEP, 5% (v/v) glycerol, pH: 7.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jul 16

Redox Modulation of Oligomeric State in Proline Utilization A. Biophys J 114(12):2833-2843 (2018)
Korasick DA, Campbell AC, Christgen SL, Chakravarthy S, White TA, Becker DF, Tanner JJ

R_g^Guinier	5.2	nm
D_max	14.2	nm
Volume^Porod	582	nm³

SASDCR3 – Proline utilization A from Legionella pneumophila 3 mg/mL

Sample:	Bifunctional protein PutA dimer, 238 kDa Legionella pneumophila subsp. … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Apr 20

...PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ

R_g^Guinier	4.6	nm
D_max	16.0	nm
Volume^Porod	291	nm³

SASDCV3 – Proline utilization A from Legionella pneumophila 5 mg/mL

Sample:	Bifunctional protein PutA dimer, 238 kDa Legionella pneumophila subsp. … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Apr 20

...PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ

R_g^Guinier	4.6	nm
D_max	15.3	nm
Volume^Porod	297	nm³

SASDCW3 – Proline utilization A from Legionella pneumophila 8 mg/mL

Sample:	Bifunctional protein PutA dimer, 238 kDa Legionella pneumophila subsp. … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Apr 20

...PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ

R_g^Guinier	4.6	nm
D_max	15.5	nm
Volume^Porod	295	nm³

SASDCX3 – Proline utilization A from Desulfovibrio vulgaris 3.0 mg/mL

Sample:	Bifunctional protein PutA dimer, 229 kDa Desulfovibrio vulgaris protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8

...PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ

R_g^Guinier	4.4	nm
D_max	16.0	nm
Volume^Porod	295	nm³

SASDCY3 – Proline utilization A from Desulfovibrio vulgaris 4.5 mg/mL

Sample:	Bifunctional protein PutA dimer, 229 kDa Desulfovibrio vulgaris protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8

...PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ

R_g^Guinier	4.4	nm
D_max	16.0	nm
Volume^Porod	294	nm³