| MWI(0) | 86 | kDa |
| MWexpected | 47 | kDa |
| VPorod | 161 | nm3 |
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log I(s)
5.60×10-2
5.60×10-3
5.60×10-4
5.60×10-5
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s, nm-1
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Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2.
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M, Nat Commun 15(1):5241 (2024) Europe PMCSASDQY5 – C-terminal binding protein 1 mutant (CtBP1: R266A, D290A, E295A, H315A)
C-terminal-binding protein 1 (R266A, D290A, E295A, H315A)|
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Fits and models
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Synchrotron SAXS
data from solutions of
C-terminal binding protein 1 mutant (CtBP1: R266A, D290A, E295A, H315A)
in
50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.2
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 6M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 0.61 mg/ml was measured
at 20°C.
40 successive
0.200 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Tetramerization mutant. |
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