A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.

Sierra-Gómez Y, Rodríguez-Hernández A, Cano-Sánchez P, Gómez-Velasco H, Hernández-Santoyo A, Siliqi D, Rodríguez-Romero A, FEBS J (2019) Europe PMC

SASDDE7 – Class I chitinase 1 from Agave tequilana

Chitinase 1
MWI(0) 31 kDa
MWexpected 32 kDa
VPorod 52 nm3
log I(s) 2.57×102 2.57×101 2.57×100 2.57×10-1
Chitinase 1 small angle scattering data  s, nm-1
ln I(s)
Chitinase 1 Guinier plot ln 2.58×102 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Chitinase 1 Kratky plot 1.104 0 √3 sRg
p(r)
Chitinase 1 pair distance distribution function Rg: 2.6 nm 0 Dmax: 9.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Chitinase 1 DAMMIF model
Synchrotron SAXS data from solutions of Class I chitinase 1 from Agave tequilana in MES 50 mM, pH 6 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring (Menlo Park, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 1.127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.23 mg/ml was measured at 15°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames = UNKNOWN

Chitinase 1 (ChiAt1)
Mol. type   Protein
Organism   Agave tequilana
Olig. state   Monomer
Mon. MW   31.8 kDa
Sequence   FASTA